BMI Seminar // Harnessing microfluidics in few-cell and single-cell manipulations for biological discovery
Event details
Date | 28.06.2017 |
Hour | 12:15 › 13:15 |
Speaker | Angela Wu, Division of Life Science, HKUST - The Hong Kong University of Science and Technology, China |
Location | |
Category | Conferences - Seminars |
Microfluidic manipulation in small fluid volumes can both increase assay sensitivities and improve reproducibility through automation. In my work, I apply microfluidics to single cell analysis, I systematically evaluate the sensitivity, precision, and accuracy of various approaches to single-cell RNA-seq. Our results show that it is possible to use single-cell RNA-seq to perform quantitative transcriptome measurements of single cells, and that microfluidic volumes help to reduce result bias. We subsequently used microfluidic-based RNA-seq to profile single cells of the developing lung in embryonic mice, as well as cells of primary human colon and cervix samples, and identified novel transcriptional markers for various subpopulations.
Practical information
- Informed public
- Free
Organizer
- SV BMI Host : H. Lashuel