BioE COLLOQUIA SERIES: "Biological Imaging at High Spatiotemporal Resolution"
Event details
| Date | 07.10.2019 |
| Hour | 12:15 |
| Speaker | Prof. Hari Shroff, National Institute of Biomedical Imaging and Bioengineering (NIBIB-NIH), Bethesda, MD (USA) |
| Location | |
| Category | Conferences - Seminars |
WEEKLY BIOENGINEERING COLLOQUIA SERIES
(sandwiches served)
Abstract:
I will discuss our efforts to improve structured illumination microscopy (SIM) and light-sheet microscopy. SIM doubles the spatial resolution of light microscopy, requiring lower light intensities and acquisition times than other super-resolution techniques. I will present SIM implementations that enable resolution doubling in live volumes > 10-20x thicker than possible with conventional SIM, as well as hardware modifications that enable effectively ‘instant’ SIM imaging at rates 10-100x faster than other SIM. Newer applications of instant SIM, including combination with total internal reflection (TIRF) and with adaptive optics will also be discussed.
The second half of the talk will focus on the development of dual-view inverted selective plane illumination microscopy (diSPIM), and subsequent application to the noninvasive study of neurodevelopment in nematodes. Newer multiview results with more objectives and more views, further improving spatial resolution, will also be shown. Applications of these technologies will be presented, including computational methods for untwisting worm embryos, and calcium and behavioral imaging of moving embryos. Finally, unpublished methods for rapidly processing very large, TB-scale light-sheet datasets will be presented.
Bio:
Dr. Hari Shroff received a B.S.E. in bioengineering from the University of Washington in 2001, and under the supervision of Dr. Jan Liphardt, completed his Ph.D. in biophysics at the University of California at Berkeley in 2006 . He spent the next three years performing postdoctoral research under the mentorship of Eric Betzig at the Howard Hughes Medical Institute's Janelia Farm Research Campus where his research focused on development of photactivated localization microscopy (PALM), an optical superresolution technique. Dr. Shroff is now chief of NIBIB's Section on High Resolution Optical Imaging laboratory, where he and his staff are developing new imaging tools for application in biological and clinical research.
Zoom link for attending remotely: https://epfl.zoom.us/j/118303710
(sandwiches served)
Abstract:
I will discuss our efforts to improve structured illumination microscopy (SIM) and light-sheet microscopy. SIM doubles the spatial resolution of light microscopy, requiring lower light intensities and acquisition times than other super-resolution techniques. I will present SIM implementations that enable resolution doubling in live volumes > 10-20x thicker than possible with conventional SIM, as well as hardware modifications that enable effectively ‘instant’ SIM imaging at rates 10-100x faster than other SIM. Newer applications of instant SIM, including combination with total internal reflection (TIRF) and with adaptive optics will also be discussed.
The second half of the talk will focus on the development of dual-view inverted selective plane illumination microscopy (diSPIM), and subsequent application to the noninvasive study of neurodevelopment in nematodes. Newer multiview results with more objectives and more views, further improving spatial resolution, will also be shown. Applications of these technologies will be presented, including computational methods for untwisting worm embryos, and calcium and behavioral imaging of moving embryos. Finally, unpublished methods for rapidly processing very large, TB-scale light-sheet datasets will be presented.
Bio:
Dr. Hari Shroff received a B.S.E. in bioengineering from the University of Washington in 2001, and under the supervision of Dr. Jan Liphardt, completed his Ph.D. in biophysics at the University of California at Berkeley in 2006 . He spent the next three years performing postdoctoral research under the mentorship of Eric Betzig at the Howard Hughes Medical Institute's Janelia Farm Research Campus where his research focused on development of photactivated localization microscopy (PALM), an optical superresolution technique. Dr. Shroff is now chief of NIBIB's Section on High Resolution Optical Imaging laboratory, where he and his staff are developing new imaging tools for application in biological and clinical research.
Zoom link for attending remotely: https://epfl.zoom.us/j/118303710
Practical information
- Informed public
- Free
Organizer
Contact
- Institute of Bioengineering (IBI), Christina Mattsson