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VERSION:2.0
PRODID:-//Memento EPFL//
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SUMMARY:EPFL BioE Talks SERIES  "Microsecond Time-Resolved Cryo-Electron M
 icroscopy"
DTSTART:20211108T160000
DTEND:20211108T170000
DTSTAMP:20260407T111332Z
UID:cf23d9044dec71279d47dfa2d7654a7ae5d262dd2704efe0eba843c9
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Ulrich J. Lorenz\, Laboratory of Molecular Nanodynamics\
 , School of Basic Sciences\, EPFL (CH)\nWEEKLY EPFL BIOE TALKS SERIES\n\nA
 bstract:\nCryo-electron microscopy (cryo-EM) is rapidly becoming the domin
 ant method in structural biology. However\, with a time resolution of seve
 ral milliseconds\, it is frequently too slow to observe proteins in actio
 n\, whose relevant dynamics typically occur on the microsecond timescale. 
 This leaves our understanding of these nanoscale machines fundamentally i
 ncomplete. We have recently demonstrated a novel approach to time-resolved
  cryo-EM that is 1000 times faster\, affording a time resolution of just a
  few microseconds. Our method involves melting a cryo sample in situ with
  a laser beam for a duration of tens of microseconds. This allows dynamics
  of the embedded particles to occur in liquid once a suitable stimulus is
  provided\, for example by releasing a caged compound. While the dynamics 
 occur\, the heating laser is switched off at a well-defined point in time
 \, causing the sample to rapidly recool\, so that it vitrifies\, trapping 
 the particles in their transient configurations\, in which they can subse
 quently be imaged. I will describe proof-of-principle experiments that dem
 onstrate the viability of the concept. Moreover\, I will discuss new aven
 ues that our technique opens up for the study of the fast dynamics of prot
 eins.\n\nBio:\nUlrich Lorenz studied chemistry at the University of Würzb
 urg\, where he discovered his interest in molecular physics. During his Ph
 D in the group of Prof. Thomas Rizzo at EPFL\, he worked on the spectrosco
 py and dynamics of cryogenic molecular ions in the gas-phase\, which inclu
 ded a fair amount of instrument development. For his postdoctoral work in 
 the group of Prof. Ahmed Zewail at Caltech\, he switched to the field of t
 ime-resolved electron microscopy\, studying the dynamics of nanoscale syst
 ems. In 2016\, he accepted a tenure-track Assistant Professor position at 
 EPFL. His research is supported by the Swiss National Science Foundation a
 s well as the European Research Council.\n\n\nIMPORTANT NOTICE:\nAs a cons
 equence of the ongoing Covid-19 pandemic\, in-person attendance of this se
 minar is subjected to some constraints:\n\n	Maximum number of participants
  is limited to 80 (2/3 of room SV1717's nominal capacity): first come\, fi
 rst served!\n	Valid COVID certificate and ID\, required to enter the meeti
 ng room\, will be checked at the entrance\n	Face masks are mandatory for e
 veryone in the seminar room (excepted the speaker while presenting).\n\nTh
 ank you warmly for your understanding!\n\nAlternatively\, the seminar can 
 also be followed via Zoom web-streaming:\nZoom link (with one-time registr
 ation for the whole series) for attending remotely: https://go.epfl.ch/EPF
 LBioETalks\n\n\nInstructions for 1st-year Ph.D. students who are under EDB
 B’s mandatory seminar attendance rule:\nIF you are not attending in-pers
 on in the room\, please make sure to\n\n\n	send D. Reinhard a note before 
 noon on seminar day\, informing that you plan to attend the talk online\, 
 and\n	be signed in on Zoom with a recognizable user name (not a pseudonym 
 making it difficult or impossible to be identified).\n\nStudents attending
  the seminar in-person should collect a confirmation signature after the t
 alk - please print your own signature sheet beforehand (71 kB pdf availabl
 e for download here).
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://go.epfl.ch/
 EPFLBioETalks
STATUS:CONFIRMED
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