BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Memento EPFL// BEGIN:VEVENT SUMMARY:EPFL BioE Talks SERIES "Lipid Driven Nanodomains Are Fluid and In terleaflet Coupled" DTSTART:20211115T160000 DTEND:20211115T170000 DTSTAMP:20260510T091520Z UID:3f0aefdacbf62ab3918aeb2e4a733861e5abab0bb2c42d58917da791 CATEGORIES:Conferences - Seminars DESCRIPTION:Prof. Martin Hof\, J. Heyrovský Institute of Physical Chemist ry\, Academy of Sciences of the Czech Republic\, Prague (CZ)\nWEEKLY EPFL BIOE TALKS SERIES\n\nAbstract:\nIt is a fundamental question whether sphin gomyelin (SM)- and cholesterol (Chol)- driven nanodomains exist in cells a nd in model membranes. Studies on model membranes revealed SM and Chol dri ven micrometer-sized liquid-ordered domains. Although the existence of suc h microdomains has not been proven for the plasma membrane\, such lipid mi xtures have been used as a model system for ‘rafts’ [1]. On the other hand\, super resolution results indicate that the plasma membrane might or ganize into nanocompartments. However\, due to the limited resolution of t hose techniques the characterization of such nanodomains is still missing. \n\nIn this lecture\, a combination of Förster resonance energy transfer and Monte Carlo simulations (MC-FRET) [2] identifies directly 10 nm large nanodomains in liquid-disordered model membranes composed of lipid mixture s containing SM and Chol [3]. That MC-FRET approach can determine the size s and concentrations of nanodomains down to 2 nm and enables studying the nanodomain inter-leaflet coupling [4\,5]. Combining MC-FRET with solid-sta te wide-line and high resolution magic angle spinning NMR [3] as well as w ith fluorescence correlation spectroscopy [6] we demonstrate that these na nodomains containing hundreds of lipid molecules are fluid  and interleaf let coupled [3\,4].\n\nAddition of GM1 ganglioside\, a molecule which form s already at concentrations of 2% fluid nanometer sized clusters in phosph atidylcholine bilayers [7\,8]\, leads to growth of those SM/ Chol nanodoma ins while preserving the fluidity. We examine the importance of the bulky ganglioside headgroup to the nanoscopic segregation of gangliosides by red ucing the size of the headgroup from ganglioside GM1\, to ganglioside GM2 and GM3. In this way\, we identified the key molecular factors that drive nanoscopic segregation of gangliosides in lipid bilayers [9].\n\nReference s\n[1]  M. Cebecauer et al. Chem Rev\, 118\, (23)\, 11259-11297\,2018\n[2 ]  R. Šachl et al. Biophys. J.\, 101\, L60-L62\, 2011\n[3]  A. Koukalov á et al. Scientific Rep. vol. 7 p. 5460\, 2017.\n[4]  I. Vinklarek et al \, J Phys Chem. Lett 10\, 2024–2030\, 2019\n[5]  M.J. Sarmento et al Fr ontiers in cell and developmental biology\, 8\, 284\, 2020\n[6]  R. Šach l et al. J. Phys. D 49 189601\, 2016\n[7]  R. Šachl et al. BBA- Mol. Cel l Res. 1853\, 850-857\, 2015.\n[8]  M. Amaro et al. Angew. Chem. vol. 55 p. 9411-9415\, 2016.\n[9]  M.J. Sarmento et al Biophys J.\, accepted.\n\n Bio:\n• Scientific education\n   2009 Full Professor for Physical Chem istry named by the Czech President\n   2006 Doctor of Science (DSc.)\, A cademy of Sciences of the Czech Republic (CAS)\n   1999 Habilitation at the Faculty for Chemistry and Pharmacy of the Julius-Maximilians-Universit y Würzburg\n   1990 Dissertation in Physical Chemistry at the Universit y Würzburg (“with excellence (1.0)”)\; Advisor: Prof. Dr. F. W. Schne ider\n   1987 "Diplom-Chemiker" at the University Würzburg\; (“with e xcellence (1.0)”)\n• Current positions\n   5/2017- Director of the J . Heyrovský Institute of Physical Chemistry\; CAS\n   2001- Lecturer an d PhD advisor at\n   Faculty of Nature Sciences of the Palacky Universit y Olomouc (Czech Republic)\,\n   Faculties of the Charles University Pra gue\n   Faculties of the Czech Technical University in Prague\, and\n    Biological Faculty of the South Bohemian University Ceske Budejovice (C zech Republic)\n   2000- Senior Scientist at the J. Heyrovský Institute \; Start-up of own scientific group\n• Previous positions\n   2007-4/2 017 Vice-Director of the J. Heyrovský Institute\, CAS\n   2006-4/2017 H ead of the Department of Biophysical Chemistry at that Institute\n   199 7-1999 Scientist at the J. Heyrovský Institute\, CAS\n   1997-1999 Assi stant Professor at the Department of Physical Chemistry\, Würzburg\n   1996 Visiting scientist at the University of Patras\, Greece (Prof. P. Lia nos)\n   1993-1995 Liebig Fellow at the Department of Physical Chemistry /Charles University Prague (Prof. V. Fidler)\n   1991-1993 Postdoctoral Fellow at the University of North Carolina at Chapel Hill (USA) (Prof. N. L. Thompson) and University Würzburg (Prof. F. W. Schneider)\n\n\nIMPORTA NT NOTICE:\nAs a consequence of the ongoing Covid-19 pandemic\, in-person attendance of this seminar is subjected to some constraints:\n\n Maximum n umber of participants is limited to 80 (2/3 of room SV1717's nominal capac ity): first come\, first served!\n Valid COVID certificate and ID\, requir ed to enter the meeting room\, will be checked at the entrance\n Face mask s are mandatory for everyone in the seminar room (excepted the speaker whi le presenting).\n\nThank you warmly for your understanding!\n\nAlternative ly\, the seminar can also be followed via Zoom web-streaming:\nZoom link ( with one-time registration for the whole series) for attending remotely: h ttps://go.epfl.ch/EPFLBioETalks\n\n\nInstructions for 1st-year Ph.D. stude nts who are under EDBB’s mandatory seminar attendance rule:\nIF you are not attending in-person in the room\, please make sure to\n\n\n send D. Re inhard a note before noon on seminar day\, informing that you plan to atte nd the talk online\, and\n be signed in on Zoom with a recognizable user n ame (not a pseudonym making it difficult or impossible to be identified).\ n\nStudents attending the seminar in-person should collect a confirmation signature after the talk - please print your own signature sheet beforehan d (71 kB pdf available for download here). LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://go.epfl.ch/ EPFLBioETalks STATUS:CONFIRMED END:VEVENT END:VCALENDAR