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SUMMARY:EPFL Neuro Seminar //Ofer Yizhar: Optogenetic dissection of local 
 and long-range connections in prefrontal circuits
DTSTART:20220518T160000
DTEND:20220518T170000
DTSTAMP:20260916T044005Z
UID:98aae72dc4a3c25e6f988c86e90e7cf6ff552ae22099adf8b4b54343
CATEGORIES:Conferences - Seminars
DESCRIPTION:Ofer Yizhar\, Weizmann Institute of Science\, Rehovot\, Israel
 \nHybrid!\n\nThe prefrontal cortex (PFC) plays an important role in regula
 ting multiple cognitive functions in mammals\, and impairments in this reg
 ion have been linked with behavioral dysfunction in psychiatric disorders.
  Neurons in the PFC connect with multiple brain-wide networks regulating b
 ehavior\, and their long-range connections to different cortical and subco
 rtical targets are thought to be involved in distinct behavioral functions
 . Specifically\, long-range outputs from the PFC to the amygdala are known
  to be involved in emotional learning\, social behavior and anxiety. I wil
 l describe a set of experiments aimed at understanding the structure of sy
 naptic connectivity among amygdala-projecting neurons in the mPFC. Using s
 ingle-neuron two-photon optogenetic stimulation and imaging\, we demonstra
 ted that these neurons form distinct connectivity modules in the deep and 
 superficial layers of the mPFC. I will then descibe the engineering of a n
 ew class of optogenetic tools for silencing of long-range axonal projectio
 ns between brain regions. I will focus on the development of eOPN3\, a lig
 ht-activated G-protein coupled receptor that couples to the inhibitory Gi/
 o signaling pathway and strongly suppresses synaptic release in vitro and 
 in vivo. eOPN3 allows specific manipulation of long-range axonal connectio
 ns\, and can be used in behaving animals to understand the role of specifi
 c network connections in cognitive function and behavior.\n 
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://epfl.zoom.u
 s/j/62550191552?pwd=OFRDVzBHb0cyVG1wYkxUSFFEMUNPdz09
STATUS:CONFIRMED
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