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PRODID:-//Memento EPFL//
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SUMMARY:EPFL BioE Talks SERIES  "Investigating Cellular Logistics With Liv
 e-Cell STED Super-Resolution Microscopy"
DTSTART:20220425T160000
DTEND:20220425T170000
DTSTAMP:20260509T203652Z
UID:344d15684d6b6f9802659edfb1aaf170e6cc3dcec21949317a6728d7
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Francesca Bottanelli\, Membrane trafficking Lab\, Instit
 ute for Chemistry and Biochemistry\, Freie Universität Berlin (D)\nWEEKL
 Y EPFL BIOE TALKS SERIES\n\nAbstract:\nIn the lab we use gene editing and 
 live-cell super-resolution microscopy to investigate the mechanisms of mem
 brane homeostasis in health and disease. To be able to image physiological
  molecular processes in the crowded cellular cytoplasm in living cells\, w
 e developed labelling methods for stimulated emission depletion (STED) sup
 er-resolution imaging in living cells and a pipeline for the rapid generat
 ion of CRISPR-Cas9 knock ins. These methods unlocked the possibility to im
 age dynamics at sub-50 nm spatial resolution and under near-native cellula
 r conditions. Dynamic nanoscale microscopy of endogenously tagged machiner
 y is revealing novel cellular roles for ARF GTPases\, one of the major fam
 ily of regulators of cellular membrane homeostasis. Defining the role of A
 RFs led to the discovery of unexplored sorting mechanisms between the endo
 plasmic reticulum and the Golgi apparatus. We show that bi-directional tra
 nsport between the ER and the Golgi apparatus occur via a dynamic and quic
 kly remodelling network of connecting nano-tunnels. In a similar way\, Gol
 gi-derived sorting tubules serve as a sorting station between Golgi and en
 do-lysosomal organelles to coordinate secretory and lysosomal trafficking 
 via kiss and run events.\n\nBio:\n2018 - present\n    Assistant Profess
 or\, Freie Universität Berlin\, Institute for Chemistry and Biochemistry\
 n2011 - 2018\n    Postdoctoral fellow\, Yale University school of medi
 cine\, Department of Cell Biology\, New Haven\, CT (USA)\n    Advisor:
  James E. Rothman\n2006 – 2011\n    PhD\, Faculty of Biological Scie
 nces\, The University of Leeds (UK)\n    Advisor: Dr. Jürgen Denecke\
 n2004 – 2006\n    MSc in Plant\, Food and Environmental Biotechnolog
 y\, University of Milan (IT)\n2001 – 2004\n    BSc in Plant Biotechn
 ology\, University of Milan (IT)\n\n\nZoom link (with one-time registratio
 n for the whole series) for attending remotely: https://go.epfl.ch/EPFLBio
 ETalks\n\n\nInstructions for 1st-year Ph.D. students who are under EDBB’
 s mandatory seminar attendance rule:\nIF you are not attending in-person i
 n the room\, please make sure to\n\n	send D. Reinhard a note before noon o
 n seminar day\, informing that you plan to attend the talk online\, and\n	
 be signed in on Zoom with a recognizable user name (not a pseudonym making
  it difficult or impossible to be identified).\n\nStudents attending the s
 eminar in-person should collect a confirmation signature after the talk - 
 please print your own signature sheet beforehand (71 kB pdf available for 
 download here).
LOCATION:BM 5202 https://plan.epfl.ch/?room==BM%205202 https://go.epfl.ch/
 EPFLBioETalks
STATUS:CONFIRMED
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