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SUMMARY:Investigating and controlling mRNA via chemo-enzymatic modificatio
 ns
DTSTART:20220524T161500
DTEND:20220524T173000
DTSTAMP:20260928T064442Z
UID:84f12ba2314597f340f2c70e8e63ce9407ce7044c3eea6e3c6e9fc72
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Andrea Rentmeister\, Univ. Münster\nMethylation of DNA\
 , RNA and proteins constitutes a major regulatory mechanism of biological 
 processes. In nature\, S‑adenosyl-l-methionine (AdoMet) is the cosubstra
 te of most methyltransferases (MTases) and the main methyl source. Synthet
 ic AdoMet analogues are also accepted by a number of promiscuous MTases or
  engineered variants.\nIn this talk\, I will show how AdoMet analogs in co
 mbination with suitable MTases can be used to transfer clickable or photo-
 cleavable (PC) groups to DNA\, RNA and the mRNA 5' cap.[1] Post-synthetic 
 bioconjugation makes MTase target sites in mRNA detectable via next genera
 tion sequencing.[2] Light-induced removal provides methodology for site-sp
 ecific “writing” and “erasing” marks on long DNA or RNA at natural
  MTase sites.\nAs a result of protein engineering of methionine adenosyltr
 ansferase (MAT)\, enzymatic in situ generation of AdoMet analogs with PC-g
 roups from their amino acid precursors is now possible.[3] The enzymatic c
 ascade accepting photocaging groups bears potential for future cellular ap
 plications.\n\nMeeting ID: 683 0972 8656\nPasscode: 974255
LOCATION:https://epfl.zoom.us/j/68309728656
STATUS:CONFIRMED
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