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SUMMARY:Notch and Foxi3 signaling dynamics modulates cell-type composition
  in the embryonic epidermis of zebrafish
DTSTART:20220627T110000
DTEND:20220627T120000
DTSTAMP:20260511T105537Z
UID:2dda1b9eb5d35f8e05795da48bbe13425423b38cd846bfdebd5e5308
CATEGORIES:Conferences - Seminars
DESCRIPTION:Bo-Kai Liao\, Assistant Professor\, Department of Aquaculture\
 , National Taiwan Ocean University\, Taiwan\nComplex body structures emerg
 e from patterned embryonic tissues. How these embryonic patterns are thems
 elves generated is a fundamental question of biology\, with implications f
 or the transformation of body structure throughout evolution and the devel
 opment of inherited disease. The skin of zebrafish embryos scattered multi
 type of ionocytes\, which are specialized cells scattered on epithelia and
  fish gills for maintaining ionic homeostasis\, and five sub-types were id
 entified in zebrafish by their physiological properties and ion channel/tr
 ansporter markers. It is suggested that the ionocyte patterning process in
 volves the lateral inhibition by Notch signaling and the downstream positi
 ve regulatory loop of foxi3a/b to determine the cell fates. However\, ther
 e is still a lack of the comprehensive lineage map to the differentiation 
 paths\, and there are few researches on the temporal control and dynamics 
 to this Notch-Foxi3 signaling axis due to technical challenges. By reanaly
 zing a deltaC transgenic reporter line\, we first discovered the there wer
 e at least 2-wave deltaC expression within a short time to divide sub-line
 age lines of ionocytes after 2~3 cell divisions. To further reveal the dif
 ferentiation trajectories of skin ionocytes and the temporal-spatial dynam
 ics\, we generated a foxi3b::foxi3b-venus\, foxi3a::foxi3a-mKate2 double t
 ransgenic zebrafish line\, named AkBash. AkBash recapitulated temporal exp
 ression patterns of the endogenous foxi3b. Surprisingly\, we discovered th
 at AkBash lost all NaRCs (Na+-K+-ATPase-rich cells) and NCCs (Na+-Cl- cotr
 ansporter cells) but showed significantly more HRCs (H+ pump-rich cells) d
 etoured from the NaRC lineage. Moreover\, a simplified modeling on 2D cell
  grids was conducted. We found that distinct cell types could be generated
  simultaneously in ionocyte progenitors\, given that both the expression a
 nd degradation rates were higher from one foxi3 to the other. Moreover\, t
 he cell types became more uniform by matching the expression rates of both
  foxi3\, as expected in AkBash. In summary\, the ionocyte progenitor patte
 rning in zebrafish are coordinated with multi-waves for Notch signaling an
 d cell divisions\, and the following sub-type determinations are sensitive
  to the temporal dynamics and the balance of the regulatory loop between f
 oxi3b and foxi3a\, which further influences the composition of ionocytes.
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://epfl.zoom.u
 s/j/64949349426?pwd=bmpXcllxVFNqVGMxRnN6ZWFBQjNjUT09
STATUS:CONFIRMED
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