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SUMMARY:Exploring transcription factor interactions using cell-free genomi
 cs
DTSTART:20230316T100000
DTEND:20230316T110000
DTSTAMP:20260407T185235Z
UID:61d581b8c49a4841990d5d9c3ae9aa07949200f275814735a1b944b8
CATEGORIES:Conferences - Seminars
DESCRIPTION:Nikolas Eggers\nPost-Doc in Peter B. Beckers lab\, Biomedical 
 Center - Molecular Biology\, LMU Munich\nAbstract: \n\nTranscription fact
 ors (TF) need to identify their physiological targets from a large pool of
  similar but ultimately nonfunctional sites. Additionally\, they need to c
 ompete with nucleosomes and other TFs for their binding sites on DNA. We e
 mploy a genome-wide in vitro chromatin reconstitution system based on pre-
 blastoderm embryonal extracts (DREX) to understand the fundamental princip
 les of TF cooperativity and binding site selection in a tightly controlle
 d cell-free system. As a model we are using the components of the Drosoph
 ila Dosage Compensation complex (DCC). The binding sites of MSL2\, the DN
 A binding subunit of the DCC\, are known. However\, the mechanisms by whic
 h they are chosen remain unresolved. By titration of additional factors an
 d competitors we were able to establish certain DNA-shape features that he
 lp to exclude other factors from MSL2`s physiological binding sites aiding
  in complex targeting. Additionally\, we used the DREX system to illustrat
 e how certain TFs can interact with nucleosomes and position them to disti
 nct positions around their binding sites.\n\nBio:\nNikolas obtained his Ba
 chelor's and Masters's degree in Biochemsitry from the University of Tu
 ebingen. He then obtained a PhD in Peter B. Beckers lab at the Ludwig-Maxi
 milians-University in Munich\, where he assayed the targeting of transcri
 ption factors to chromatin using an in vitro chromatin reconstitution syst
 em. Nikolas is now working as a Post-Doc in Peter B. Beckers lab\, again 
 working on transcription factor targeting\, nucleosome interaction and o
 ther chromatin related topics.
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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