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SUMMARY:Localizomics: spatial omics using DNA-based super-resolution micro
 scopy
DTSTART:20221116T110000
DTEND:20221116T120000
DTSTAMP:20260407T103345Z
UID:684eccff7f47806e81c22e8cc4701ffbe7127ee08fd7fefd9c65ea85
CATEGORIES:Conferences - Seminars
DESCRIPTION:Ralf Jungmann\, Max Planck Institute of Biochemistry\, Munich\
 nSuper-resolution fluorescence microscopy is a powerful tool for biophysic
 al and biological research. We use the transient binding of short fluoresc
 ently labeled oligonucleotides (DNA-PAINT) for easy-to-implement multiplex
 ed super-resolution imaging that achieves molecular-scale resolution.\nIn 
 this talk\, I will discuss our group’s vision of enabling standard off-t
 he-shelf fluorescence microscopy hardware to become a tool for spatial omi
 cs. This will allow researchers to visualize most biomolecules and probe t
 heir network-wide interactions in single cells\, tissues\, and beyond with
  single-molecule localization microscopy: Localizomics.\nTo enable this\,
  I will first introduce major technical advancements in DNA-PAINT includin
 g approaches that achieve sub-10-nm spatial resolution and spectrally unli
 mited multiplexing in whole cells. I will then discuss novel protein label
 ing probes such as Slow Off-rate Modified Aptamers (SOMAmers) that are poi
 sed to allow DNA-barcoded labeling of much of the proteome in an intact ce
 llular environment.\nNext\, I will discuss efforts to substantially increa
 se DNA-PAINT’s traditionally relatively slow image acquisition speeds to
  achieve experimental throughputs necessary for network-wide molecular int
 errogation. To this end\, we employed rationale\, de novo DNA sequence des
 ign and repetitive sequence motifs and demonstrate in vitro and in situ im
 aging achieving 100-times faster imaging speeds without compromising image
  quality or spatial resolution. This improvement now makes DNA-PAINT appli
 cable to high-throughput studies with biomedical relevance.\nFinally\, I w
 ill present first applications of these new capabilities to cell surface r
 eceptor imaging and quantification at thus far elusive spatial resolutions
  and levels of multiplexing. This is poised to yield fundamental insights 
 into the molecular architecture of surface receptor interactions and poten
 tially enable the future development of more refined “pattern”-based t
 herapeutics.\n 
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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