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PRODID:-//Memento EPFL//
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SUMMARY:Scanning Ion Conductance Microscopy
DTSTART:20230209T170000
DTEND:20230209T180000
DTSTAMP:20260406T213006Z
UID:345afc40a3cef1190cb7e9144a1e84a0b1af194e29a32804c2f2250d
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Georg Fantner and Prof. Aleksandra Radenovic\, EPFL \nA
 bstract:  \nNanopores are one of the most successful label-free single-m
 olecule techniques with several sensing applications such as biological sc
 reening\, diagnostics\, DNA and protein sequencing. In current nanopore te
 chnologies\, stochastic processes influence both the selection of the tran
 slocating molecule\, translocation rate and translocation velocity. As a r
 esult\, single-molecule translocations are difficult to control spatially 
 and temporally. In this talk we will present a novel method where we engin
 eered precise spatial and temporal control into the single-molecule experi
 ment. We used a glass nanopore mounted on a 3D nanopositioner to spatially
  select molecules\, deterministically tethered on a glass surface\, for co
 ntrolled translocations. By controlling the distance between the nanopore 
 and the glass surface\, we could actively select the region of interest on
  the molecule and scan it a controlled number of times and at controlled v
 elocity. Decreasing the velocity and averaging thousands of consecutive re
 adings of the same molecule increases the signal-to-noise ratio (SNR) by t
 wo orders of magnitude compared to free translocations. We applied our met
 hod to various DNA constructs\, achieving down to single nucleotide gap re
 solution. The spatial multiplexing combined with the sub-nanometer resolut
 ion could be used in conjunction with micro-array technologies to enable s
 creening of DNA\, improving point of care devices\, or enabling high-densi
 ty\, addressable DNA data storage.\n\nBiographies:  \n\nProf. Georg Fant
 ner received the Ph.D. degree in physics from the University of California
 \, Santa Barbara\, Santa Barbara\, CA\, USA\, in 2006. He was an Erwin Sch
 rödinger Fellow with the Massachusetts Institute of Technology\, Cambridg
 e\, MA\, USA\, for three years. He then joined the Faculty at the École P
 olytechnique Fédérale (EPFL)\, Lausanne\, Switzerland. As an Associate P
 rofessor\, he heads the Laboratory for Bio- and Nano-Instrumentation\, Int
 erfaculty Institute for Bioengineering\, and the Microengineering Institut
 e. He has authored many internationally recognized papers and book chapter
 s. His current research interests include developing next generation nanos
 cale measurement technologies for life science. Dr. Fantner was a recipien
 t of an ERC Starting Grant for 2012–2017 and an ERC Consolidator Grant f
 or 2018–2023. He served as a Guest Editor for the International Journal 
 of Molecular Sciences. He serves as an Editor for Microscopy and Microanal
 ysis (Cambridge University Press) and an Editorial Board Member of Scienti
 fic Reports (Nature Publishing Group). \n\nProf. Aleksandra Radenovic is 
 a full professor of biological engineering at the École Polytechnique Fé
 dérale de Lausanne (EPFL) and head of the Laboratory of Nanoscale Biology
 . Her lab works in the research field that can be termed single-molecule b
 iophysics. She has received her Ph.D. in Biophysics from the University of
  Lausanne (Switzerland.) in 2003 and a Msc. in Physics from the University
  of Zagreb (Croatia) in 2000. In 2010\, she received a European Research C
 ouncil (ERC) Starting Grant in 2010 and SNF Backup scheme Consolidator Gra
 nt (2015). She is also the recipient of the CCMX materials challenge award
  in 2016 and the Advanced ERC (2020) grant. She develops techniques and me
 thodologies based on optical imaging\, bio-sensing and single-molecule man
 ipulation with the aim to monitor the behavior of individual biological mo
 lecules and complexes in vitro and in live cells.
LOCATION:BC 420 https://plan.epfl.ch/?room==BC%20420
STATUS:CONFIRMED
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