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SUMMARY:Micropipette-based tools to probe cellular and intracellular mecha
 nics: from immune cell activation to cell adhesion and rupture\, to the me
 chanics of mitochondrial fission
DTSTART:20230905T160000
DTEND:20230905T170000
DTSTAMP:20260408T090724Z
UID:7d3c0b8c07ebebb2e5f3a2c3bcdab39dcda3460764650c0873c85c3b
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Julien Husson\nLadHyX\, CNRS\, Ecole polytechnique\, Ins
 titut polytechnique de Paris\, route de Saclay\,\nF - 91128 Palaiseau Cede
 x\nProbing single-cell mechanics requires sensitive force probes. Although
  established techniques such as optical tweezers and atomic force microsco
 py are powerful tools\, when performing cellular-scale studies\, they do n
 ot systematically cover the relevant range of forces or stiffness\, nor do
  they always provide enough versatility to allow observing the cells while
  probing them. This leaves room for custom-made and cost-effective approac
 hes. Here I will start by briefly describing various techniques that we ha
 ve developed to probe the mechanics of adherent and non-adherent cells. Th
 ese techniques are based on micropipettes\, a tool that\, although introdu
 ced almost a century ago\, still allows for development and provides a pow
 erful tool for quantitative measurements. Using these tools\, we have meas
 ured forces generated by immune cells during activation and shown that leu
 kocytes become stiffer during activation. Furthermore\, by aspirating adhe
 rent cells\, we could quantify cell-substrate adhesion\, and by compressin
 g adherent cells with controlled forces\, we could establish a criterion f
 or membrane rupture. In a second part\, I will explain how\, by rupturing 
 the plasma membrane of a cell using micropipettes\, we could induce an imm
 ediate fission of all mitochondria within this cell\, not unlike what occu
 rs during cell infection by specific toxins. By modeling mitochondrial mec
 hanics using fluid mechanics approaches\, we could explain the typical siz
 e of mitochondria after fission and the role of mitochondrial morphology i
 n the occurrence of fission events
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://epfl.zoom.u
 s/j/69838993974
STATUS:CONFIRMED
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