BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Memento EPFL// BEGIN:VEVENT SUMMARY:Open Seminar 26.09.23 16:15\, prof. Sarah D’Adamo\, Netherlands "Solar lipid production by oleaginous microalgae: revisiting lipid metabol ism and tailoring lipid profile through genetic engineering" DTSTART:20230926T161500 DTEND:20230926T173000 DTSTAMP:20260428T005500Z UID:4354e3d5809168a404ffec96b7b86bd3da09205a468afe2c6b73ef64 CATEGORIES:Conferences - Seminars DESCRIPTION:Welcome to the open seminar of Invited Professor Sarah D'Adamo .\n \nTuesday September 26\, 16:15\nprof.Sarah D'Adamo\nWageningen Unive rsity and Research\, The Netherlands\n\nTitle: Solar lipid production by oleaginous microalgae: revisiting lipid metabolism and tailoring lipid pr ofile through genetic engineering"\n \nThis seminar will be held in perso n\nat EPFL Lausanne in the room CH B3 31\nfollowed by an Apéro and Q&A s ession at 17:30\n \nZOOM link https://epfl.zoom.us/j/84209090880\n \nAb stract:  \nSolar lipid production by oleaginous microalgae: revisiting l ipid metabolism and tailoring lipid profile through genetic engineering\nS arah D’Adamo1\, Christian Südfeld1\, René Wijffels1 and Maria Barb osa1\n1Bioprocess Engineering & AlgaePARC\, Wageningen University and Rese arch\, P.O. Box 16\, 6700 AA Wageningen\, The Netherlands\n \nMicroalgae have caught industrial interest as promising sustainable photosynthetic pr oduction platforms for several compounds (i.e.\, proteins\, hydrocarbons\, fatty acids) exploitable in food and feed\, aquaculture\, and fuel sector s. However\, in order to become an economically viable platform\, these mi croorganisms must be optimized in solar energy conversion\, carbon capture and utilization\, and the partitioning of metabolic fluxes (1\,2). To ful fil the necessity of improved yields in microalgal lipid production\, our research moves towards the genetic domestication of robust\, oleaginous mi croalgae\, such as Nannochloropsis oceanica. Part of our very recent acti vities involves the development of novel tools for both gene editing and g ene expression.  Therefore\, in this talk\, we present our recent develo pments on cutting-edge gene editing tools by using CRISPR-Cas systems (3\, 4)\, and a new developed gene expression system based on RNA polymerase I activity (3). We report how we have successfully employed high-throughput screening techniques to identify and to select new mutant lines (5\, 6\, 7 ). We finally discuss our recent targeted genetic engineering activities a imed to generate and investigate high-lipid phenotypes\, and to tailor lip id composition of N. oceanica\, towards the inclusion of novel lipid clas ses (8\,9). \n \nReferences\n1.      Work VH\, D’Adamo S\, Radak ovits R\, Jinkerson RE\, Posewitz MC. Improving photosynthesis and metabol ic networks for the competitive production of phototroph-derived biofuels.  Curr Opin Biotechnol. 2012\;23(3):290-297. doi:10.1016/j.copbio.2011.11. 022\n2.      Remmers IM\, D’Adamo S\, Martens DE\, et al. Orchest ration of transcriptome\, proteome and metabolome in the diatom Phaeodacty lum tricornutum during nitrogen limitation. Algal Res. 2018\;35(July):33- 49. doi:10.1016/j.algal.2018.08.012\n3.      Ibnu M\, Naduthodi S\, Mohanraju P\, Südfeld C\, D'Adamo S\, Barbosa MJ\, J van der Oost. CRISP R – Cas ribonucleoprotein mediated homology ‑ directed repair for effi cient targeted genome editing in microalgae Nannochloropsis oceanica IMET1 . Biotechnol Biofuels 2019\; 1–11. https://doi.org/10.1186/s13068-019-14 01-3\n4.      Naduthodi MIS\, Südfeld C\, Avitzigiannis EK\, D'Adam o S\, Barbosa MJ\, J van der Oost Comprehensive Genome Engineering Toolbox for Microalgae Nannochloropsis oceanica Based on CRISPR-Cas Systems. ACS Synth Biol. 2021\;10(12):3369-3378. doi:10.1021/acssynbio.1c00329\n5.       Südfeld C\, Pozo-Rodríguez A\, Manjavacas Díez SA\, Wijffels RH \, Barbosa MJ\, D’Adamo S. The nucleolus as a genomic safe harbor for st rong gene expression in Nannochloropsis oceanica. Plant cell\, Volume 15\ , Issue 2\, 2022\, Pages 340-353 https://doi.org/10.1016/j.molp.2021.11.00 3\n6.      Südfeld C\, Hub M\, D'Adamo S\, Wijffels RH\, Barbosa MJ . Optimization of high-throughput lipid screening of the microalga Nannoc hloropsis oceanica using BODIPY 505 / 515. Volume 53\, March 2021\, 10213 8\, https://doi.org/10.1016/j.algal.2020.102138\n7.      Südfeld C\ , Hubáček M\, Figueiredo D\, Naduthodi MIS\, van der Oost J\, Wijffels R H\, Barbosa MJ\, D'Adamo S. High-throughput insertional mutagenesis reveal s novel targets for enhancing lipid accumulation in Nannochloropsis oceani ca. Metab Eng. 2021\;66(May):239–58\n8.      Südfeld C\, Kiyani A \, Buckens H\, Hubáček M\, Wijffels RH\, Barbosa MJ\, D'Adamo S. Accumul ation of medium chain fatty acids in Nannochloropsis oceanicaby heterolog ous expression of Cuphea palustris thioesterase FatB1 Algal Research Vol ume 64\, May 2022\, 102665\, https://doi.org/10.1016/j.algal.2022.102665\n 9.      Südfeld C\, Kiyani A\, Wefelmeier K\, Wijffels RH\, Barbosa MJ\, D'Adamo S Expression of glycerol-3-phosphate acyltransferase increas es non-polar lipid accumulation in Nannochloropsis oceanica\n10.    Mi crobial Cell Factories volume 22\, Article number: 12 (2023) https://doi.o rg/10.1186/s12934-022-01987-y\n \n Presenting author\n  \n Sarah D’ Adamo\nAssistant professor I\nWageningen University and Research\nThe Neth erlands\n* sarah.dadamo@wur.nl\nhttps://nl.linkedin.com/in/sarah-d-adamo-a 1ba8b\n \n \nBIOGRAPHY\nSarah D’Adamo holds a MSc. in Industrial biot echnology and PhD degree in biotechnology\, curriculum: biochemistry and b iophysics\, both obtained at the University of Study of Padova\, Italy. Fr om January 2018\, she is a tenure-track Assistant Professor in microalgal cell factory design at the Bioprocess Engineering division of Wageningen U niversity (WUR)\, The Netherlands. She worked as post-doctoral fellow and research associate at the Chemistry Dept.ment of Colorado School of Mines\ , United States of America\, following projects on redox and hydrogen meta bolism of green microalgae. She has been senior scientist and lead scienti st at Algenuity\, a microalgal biotech start-up company based in England. Overall\, she has >12 years’ of both academic and industrial experience in the field of microalgae physiology and biotechnology\, conducted in Ita ly\, USA\, UK and\, NL. Expertise includes analytical chemistry\, biochemi stry\, molecular biology\, biophysics\, redox reactions\, photosynthesis\, metabolic engineering\, strain engineering\, bioprocess design\, nutraceu tical production. \n \nWageningen University and Research (WUR) is a co llaboration of Wageningen University and specialized research institutes o f the Wageningen Research foundation\, which have joined forces `to explor e the potential of nature to improve the quality of life´. The Bioprocess Engineering division (BPE) of WUR is performing research on the developme nt of novel biotechnological processes for production of bio-based chemica ls\, biofuels\, food additives\, feed and pharmaceuticals. WUR-BPE’s ch allenge is to produce high quality biobased products in a sustainable and economical way. WUR-BPE has over 15 years’ experience with the cultivati on of algae and has a solid scientific basis in the expertise of bioreacto r design\, medium optimization\, strain improvement\, scale-up\, biorefine ry and LCA’s in this area of application. 25 PhD students\, 2 professors \, 2 assistant professors and 2 PostDocs from our staff are working in mic roalgae field. We collaborate already with over 40 companies and we coordi nate large public private partnership programs (AlgaePARC pilot facilities \, AlgaePARC Biorefinery and 3 EU research programs).  LOCATION:CH B3 31 https://plan.epfl.ch/?room==CH%20B3%2031 https://epfl.zo om.us/j/84209090880 STATUS:CONFIRMED END:VEVENT END:VCALENDAR