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SUMMARY:Imaging Seminar: Correlative cryoSEM-CryoNanoSIMS mapping of vitri
 fied biological tissue
DTSTART:20240425T170000
DTEND:20240425T180000
DTSTAMP:20260415T163905Z
UID:782476214fece9089dd1a52bf63e9a768cdd8f177f749b71649d8655
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr. Priya Ramakrishna\, EPFL\njoint seminar with the Dubochet
  Center for Imaging \n\n<< Registration below >>\n\nAbstract:\nNanosca
 le Secondary Ion Mass Spectrometry (NanoSIMS) is an ion microprobe based i
 maging technique that produces quantified maps of chemical and isotopic va
 riations in a wide range of materials with high lateral resolution (typica
 lly 100 nm). NanoSIMS is often used to understand complex transport and me
 tabolic processes in living organisms. However\, a big challenge has been 
 to preserve the samples' natural state during analysis. The recent develop
 ment of a CryoNanoSIMS by Prof. Anders Meibom and team at EPFL now enables
  examination of vitrified biological samples\, i.e. samples prepared for a
 nalysis without loss or significant displacement of cell constituents\, in
 cluding soluble compounds. This breakthrough enables high-resolution imagi
 ng of elements in biological tissue in its most pristine state. In this se
 minar\, I will present the cryo-workflow that includes vitrification by hi
 gh pressure freezing\, cryoplaning of the sample surface\, cryoSEM imaging
  for ultrastructure and correlative CryoNanoSIMS for isotopic and chemical
  imaging of biological tissues. I will present a case study from plant dev
 elopmental biology in which the CryoNanoSIMS has been used to visualize mu
 ltiple physiologically important elements in vitrified plant roots at the 
 subcellular scale\, greatly expanding our understanding of salinity stress
  in plants\, which causes significant loss of crop-yield globally.\n\nBio:
 \nPriya Ramakrishna is a developmental biologist with an Engineering degre
 e in Biotechnology from India. She then obtained a master’s in Plant Gen
 etics\, followed by a Ph.D. in Plant Developmental Biology from the Univer
 sity of Nottingham in the UK. She is currently a Scientist in the group of
  Prof. Anders Meibom at the Laboratory of Biological Geochemistry in EPFL\
 , Switzerland. Her research is at the interface of elemental physiology an
 d plant developmental biology and she is pioneering the application of the
  newly developed CryoNanoSIMS ion microprobe on plant tissues to address t
 he issue of salinity stress and its impact on agriculture. The work is a j
 oint effort with an interdisciplinary team of researchers at LGB-EPFL\, th
 e Electron Microscopy Facility and the group of Prof. Niko Geldner at the 
 Department of Plant Molecular Biology at UNIL.\n\nRegistration appreciated
 \nMore info here
LOCATION:BM 5202 https://plan.epfl.ch/?room==BM%205202
STATUS:CONFIRMED
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