BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Memento EPFL//
BEGIN:VEVENT
SUMMARY:EPFL BioE Talks SERIES  "Advanced Concepts of Super-Resolution Flu
 orescence Microscopy"
DTSTART:20240422T121500
DTEND:20240422T131500
DTSTAMP:20260506T102715Z
UID:4f9a6e5b818891eb419ff38452c99a6c9e94952ca180835153566fbc
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Jörg Enderlein\, Third Institute of Physics – Biophys
 ics\, Georg August University\, Göttingen (DE)\nWEEKLY EPFL BIOE TALKS SE
 RIES (sandwiches provided)\n\nAbstract:\nWith the advent of super-resoluti
 on microscopy\, the last ~25 years have seen a revolution in optical micro
 scopy\, pushing the spatial resolution capabilities of optical microscopy 
 towards length scales that were typically accessible only by electron micr
 oscopy. In my presentation\, I will give a short overview of the different
  principal approaches to super-resolution microscopy. Then\, I will focus 
 on two specific techniques where our group has contributed most. The first
  is Image Scanning Microscopy or ISM [1-3]. This technique uses a simple c
 ombination of confocal microscopy with wide-field image detection for doub
 ling the resolution of conventional microscopy. I will explain the physica
 l principals behind ISM\, and the various kinds of its implementation. Mea
 nwhile\, ISM has found broad and wide applications and lies behind state-o
 f-the-art commercial systems such as the extremely successful AiryScan mic
 roscope from Carl Zeiss Jena. The second method is Metal-and Graphene-Indu
 ced Energy Transfer imaging or MIET/GIET imaging [4-6]. It addresses the a
 xial resolution in microscopy\, which is particularly important for resolv
 ing three-dimensional structures. MIET/GIET is based on the intricate elec
 trodynamic interaction of fluorescent emitters with metallic nanostructure
 s. I will present the basic principles and several applications of this te
 chnique.\n\nReferences:\n1. Müller\, C. B.\, & Enderlein\, J. (2010). Ima
 ge scanning microscopy. Phys. Rev. Lett. 104\, 198101.\n2. Schulz\, O.\, P
 ieper\, C.\, Clever\, M.\, Pfaff\, J.\, Ruhlandt\, A.\, Kehlenbach\, R. H.
 \, ... & Enderlein\, J. (2013). Resolution doubling in fluorescence micros
 copy with confocal spinning-disk image scanning microscopy. PNAS 110\, 210
 00-21005.\n3. Gregor\, I.\, Spiecker\, M.\, Petrovsky\, R.\, Großhans\, J
 .\, Ros\, R.\, & Enderlein\, J. (2017). Rapid nonlinear image scanning mic
 roscopy. Nature Methods 14\, 1087.\n4. Chizhik\, A. I.\, Rother\, J.\, Gre
 gor\, I.\, Janshoff\, A.\, & Enderlein\, J. (2014). Metal-induced energy t
 ransfer for live cell nanoscopy. Nature Photonics 8\, 124.\n5. Ghosh\, A.\
 , Sharma\, A.\, Chizhik\, A. I.\, Isbaner\, S.\, Ruhlandt\, D.\, Tsukanov\
 , R.\, ... & Enderlein\, J. (2019). Graphene-based metal-induced energy tr
 ansfer for sub-nanometre optical localization. Nature Photonics 13\, 860-8
 65.\n6. Ghosh\, A.\, Chizhik\, A. I.\, Karedla\, N.\, & Enderlein\, J. (20
 21). Graphene-and metal-induced energy transfer for single-molecule imagin
 g and live-cell nanoscopy with (sub)-nanometer axial resolution. Nature Pr
 otocols 16\, 3695-3715.\n7. Chen\, T.\, Ghosh\, A.\, & Enderlein\, J. (202
 3). Cholesterol-induced nanoscale variations in the thickness of phospholi
 pid membranes. Nano Letters 23\, 2421-2426.\n\nBio:\nJörg Enderlein has s
 tudied physics in Odessa (Ukraine) between 1981 and 1986. He obtained his 
 PhD at Humboldt-University in Berlin (Germany) in 1991 for his research on
  non-linear reaction diffusion systems. After his PhD\, he joined PicoQuan
 t GmbH in berlin as a research scientist\, where he was involved in the de
 velopment of technology for single-molecule fluorescence detection and spe
 ctroscopy. After his PostDoc with the group of Dick Keller at the Los Alam
 os National Laboratory (USA)\, he became an assistant professor at Regensb
 urg University (Germany) in 1997. In 2001\, he became a Heisenberg Fellow 
 of the German Research Council and a group leader at the Forschungszentrum
  Jülich\, Germany’s largest research institution. In 2007\, he became f
 ull professor for Biophysical Chemistry at the Eberhard Karls University i
 n Tübingen\, and since 2008\, he is full professor for Biophysics at the 
 Georg August University in Göttingen. His research interests are single-m
 olecule fluorescence spectroscopy\, super-resolution fluorescence microsco
 py\, and nano-optics and plasmonics.\n\n\nZoom link (with one-time registr
 ation for the whole series) for attending remotely: https://go.epfl.ch/EPF
 LBioETalks\n\n\nInstructions for 1st-year Ph.D. students who are under EDB
 B’s mandatory seminar attendance rule:\nIN CASE you cannot attend in-per
 son in the room\, please make sure to\n\n	send D. Reinhard a note well ahe
 ad of time (ideally before seminar day)\, informing that you plan to atten
 d the talk online\, and\, during seminar:\n	be signed in on Zoom with a re
 cognizable user name (not any alias making it difficult or impossible to i
 dentify you).\n\nStudents attending the seminar in-person should collect a
  confirmation signature after the talk - please print your own signature s
 heet beforehand (71 kB pdf available for download here). IMPORTANTLY: hang
  on to this sheet as no signature record is being kept by anyone else!
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://go.epfl.ch/
 EPFLBioETalks
STATUS:CONFIRMED
END:VEVENT
END:VCALENDAR