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SUMMARY:Multifunctional Nanopores and 2D Material Surfaces for Single-Mole
 cule Studies
DTSTART:20240523T143000
DTEND:20240523T153000
DTSTAMP:20260315T160823Z
UID:3978e01b4c40f3de183b55aa2f4c97c7b21a76d507e6091a9d5e4e9c
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Sabina Caneva\, Nanoelectromechanical Systems for Single
 -Molecule Biophysics\, Department of Precision and Microsystems Engineerin
 g\, Delft University of Technology - TU Delft (NL)\nBIOENGINEERING SEMINAR
 \n \nAbstract:\nSingle-molecule techniques are emerging as powerful tools
  to investigate the dynamics\, mechanics and interactions of biomolecules 
 with high spatial and high temporal resolution. They are increasingly used
  to answer fundamental questions in biology and are employed in next-gener
 ation sequencing for molecular diagnostics. In this talk\, I will present 
 two platforms for single-molecule studies. The first will focus on 2D mate
 rial optofluidics\, where we leverage the atomically-smooth and fluorescen
 ce compatible surfaces of hexagonal boron nitride crystals to study DNA bi
 nding and diffusion. We find that the diffusion behaviour is dependent on 
 a number of parameters including DNA sequence\, length\, hBN surface topog
 raphy and defect content\, as well as solvent conditions.\nIn the second p
 art\, I will introduce DNA origami nanoactuators as size-selective nanopor
 es enabling on-demand molecular transport across lipid bilayer membranes. 
 We leverage the structural precision of DNA origami nanotechnology with ma
 chine-inspired component design to generate structurally-adaptable nanopor
 es featuring reversible gating triggers. Using AFM\, TEM and DNA PAINT we 
 confirm the conformational changes that modulate the size of the nanopore 
 channel. Additionally\, we demonstrate localization of the actuators at th
 e membrane via confocal fluorescence imaging\, and illustrate size-selecti
 ve translocation of dextran molecules of different molecular weights via d
 ye influx assays. These fully-reconfigurable structures can be employed in
  the delivery of macromolecules\, as well as in the development of synthet
 ic cells.\n\nBio:\nSabina Caneva is a tenure track Assistant Professor at 
 TU Delft. She obtained a BSc and MSc in Materials Science from Oxford Univ
 ersity (2012) and PhD in Engineering from the University of Cambridge (201
 6). Subsequently\, she joined the Kavli Institute of Nanoscience in Delft 
 as a Marie Curie postdoctoral Fellow focusing on molecular electronics wit
 h 2D materials. In 2020\, she was awarded a Delft Technology Fellowship to
  start her independent research group at the Department of Precision and M
 icrosystems Engineering. Her group develops nanoelectromechanical systems 
 for single-molecule biophysics studies using a combination of 2D material 
 nanodevices\, optical nanoscopy and acoustofluidics.\n\n\nZoom link for at
 tending remotely: https://epfl.zoom.us/j/61246451757
LOCATION:BM 5202 https://plan.epfl.ch/?room==BM%205202 https://epfl.zoom.u
 s/j/61246451757
STATUS:CONFIRMED
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