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SUMMARY:EPFL BioE Talks SERIES  "Mechanistic Data From Ultrafast 2D Infrar
 ed Spectroscopy Leads to an Atomic Structural Model of an Amyloid Oligomer
 "
DTSTART:20250505T121500
DTEND:20250505T131500
DTSTAMP:20260405T232352Z
UID:b79deb1a7614f485a8950ada470d96c7bf960185ae29f0a00934ae23
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Martin T. Zanni\, University of Wisconsin\, Madison\, WI
  (USA)\nWEEKLY EPFL BIOE TALKS SERIES (sandwiches provided)\n\nAbstract:\n
 Obtaining the atomic structures of amyloid oligomers is one of the biggest
  outstanding problems in structural biology. Amyloid oligomers are transie
 nt species\, often present at low concentrations. As such\, they are not e
 asily amenable to traditional structural biology techniques like X-ray cry
 stallography\, NMR spectroscopy or cryoEM microscopy. If aggregation can b
 e slowed or the oligomers trapped by either stabilizing the oligomer or de
 stabilizing the fibrils\, then atomic structures have been determined\, bu
 t only in a few rare cases and under conditions that may be perturbative t
 o the natural structure. In this talk\, 2D IR spectroscopy of the human is
 let amyloid polypeptide (hIAPP) associated with type 2 diabetes will be pr
 esented. Mechanistic information on the aggregation mechanism has been obt
 ained by monitoring the kinetics from monomers to oligomers to fibrils.  
 Using isotope labeling\, a quaternary fold of the oligomer has been discov
 ered in an important region of the protein.  With that information\, alon
 gside homologous sequences between species\, a few mutations were placed t
 hat extended aggregation time from hours to days.  That enables high-reso
 lution 2D/3D NMR spectroscopy for the first time on hIAPP\, giving rise to
  more than 300 structural constraints.  Replica exchange molecular dynami
 cs generated a structure that satisfies both the 2D IR and NMR constraints
 \, providing the first oligomer model for hIAPP.  It has implications for
  drug discovery and the effects of a familial mutation that leads to early
  onset type 2 diabetes.\n\nBio:\nMartin T. Zanni is the Meloche-Bascom Pro
 fessor of Chemistry at the University of Wisconsin-Madison. He received hi
 s PhD from the University of California-Berkeley\, working with Dan Neumar
 k\, and was an NIH Postdoctoral Fellow at the University of Pennsylvania w
 ith Robin Hochstrasser. He is one of the early pioneers of 2D IR spectrosc
 opy and has made many technological innovations that have broadened the ca
 pabilities and scope of multidimensional spectroscopies and microscopies. 
 He utilizes these new techniques to study topics in biophysics\, chemical 
 physics\, photovoltaics\, and surfaces. He has received many national and 
 international accolades for his research. Notably\, he is the only person 
 to have received the ACS Nobel Laureate Signature Award as both a student 
 and a mentor and the first person to receive the Craver\, Coblentz\, and L
 ippincott Awards. He founded PhaseTech Spectroscopy Inc.\, which is the fi
 rst company to commercialize 2D IR and 2D Electronic spectroscopies.\n\n\n
 Zoom link (with one-time registration for the whole series) for attending 
 remotely: https://go.epfl.ch/EPFLBioETalks\n\n\nInstructions for 1st-year 
 Ph.D. students planning to attend this talk\, who are under EDBB’s manda
 tory seminar attendance rule:\nIN CASE you cannot attend in-person in the 
 room\, please make sure to\n\n	send D. Reinhard a note well ahead of time 
 (ideally before seminar day)\, informing that you plan to attend the talk 
 online\, and\, during seminar:\n	be signed in on Zoom with a recognizable 
 user name (not any alias making it difficult or impossible to identify you
 ).\n\nStudents attending the seminar in-person should collect a confirmati
 on signature after the talk - please print your own signature sheet before
 hand (69 kB pdf available for download here). IMPORTANTLY: hang on to this
  sheet as no signature record is being kept by anyone else!
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://go.epfl.ch/
 EPFLBioETalks
STATUS:CONFIRMED
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