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SUMMARY:EPFL BioE Talks SERIES  "Towards Better Diagnostics and Biomarkers
 : Microfluidics\, 3D Printing\, and Single Extracellular Vesicle Analysis"
DTSTART:20250428T121500
DTEND:20250428T131500
DTSTAMP:20260406T102330Z
UID:3144ba0f5e491a818abb1a61c42503455e8a2e75f0e1c7c0ad0e4e73
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. David Juncker\, Biomedical Engineering Dept. McGill Univ
 ersity\, Montreal (CAN)\nWEEKLY EPFL BIOE TALKS SERIES (sandwiches provide
 d)\n\nAbstract:\nEarly and accurate diagnostics could greatly improve heal
 th and well-being\, but are often not available when needed because of tec
 hnological barriers and lack of biomarkers. This presentation will summari
 ze our efforts in advanced microfluidics and 3D printing for point-of-care
  diagnostics\, and single extracellular vesicle (EV) analysis for uncoveri
 ng biomarkers. Capillary microfluidics can be made by 3D printing as auton
 omous systems thanks to self-powered\, pre-programmed (i.e. structurally-e
 ncoded) microfluidic circuits called capillarics. The development of very 
 low-cost\, high-resolution 3D stereolithography printing for making microf
 luidics will be described. These two advances may pave the way for distrib
 uted digital manufacturing of rapid diagnostics at the point-of-need. Resu
 lts in the design\, operation\, and manufacturing of capillarics for point
 -of-care diagnostics\, will be shown\, including with very low cost ($300)
  LCD 3D printers\, that open the door for anyone anywhere to make their ow
 n. Next\, EVs\, small lipid vesicles between ~ 40 – 400 nm will be discu
 ssed and their potential as biomarkers explained\, especially if measured 
 individually. Yet bulk analysis of EVs remains favoured because of technic
 al limitations. We introduced a new method for single EV analysis called s
 ize photometry and fluorescence imaging (SPFI) for the high throughput\, c
 oncomitant label-free sizing and fluorescence imaging of single EVs on a c
 ommon fluorescence microscope. The use of SPFI with barcoded antibodies op
 ens the door to high multiplex affinity proteomics of single EVs. Proof-of
 -concept studies on cell line EVs\, EVs spiked into plasma\, and patient s
 amples uncovering EV biology and potential biomarkers will be shown.\n\nBi
 o:\nDavid Juncker holds a Canada Research Chair in Bioengineering and is a
  Professor in the department of Biomedical Engineering at McGill Universit
 y in Montreal\, while also currently serving as department chair. David ob
 tained a Diploma (1995)\, and PhD (2002) from the Institute of Microtechno
 logy (now part of EPFL) at the University of Neuchâtel (CH) for research 
 conducted at the IBM Zurich Research Laboratory. David started as an assis
 tant professor at McGill in 2005.\nDavid’s group’s research is a mix o
 f grand challenges and of curiosity-driven interdisciplinary efforts in bi
 oengineering including microfluidics\, microfabrication\, imaging\, diagno
 stics\, assay multiplexing\, biomarker discovery\, and organ-on-a-chip. So
 me of the highlights of the lab’s accomplishments include the ideation a
 nd realization of capillary microfluidic systems and capillaric circuits c
 ulminating in microfluidic chain reactions and the ELISA-chip\; microfluid
 ic probes for microfluidics without microchannels\; affordable\, scalable 
 cross-reactivity free multiplexed sandwich immunoassays thanks to antibody
  colocalization microarrays and colocalization-by-linkage assays on microp
 articles. 3D printing (DLP & LCD) and digital manufacturing of functional 
 microfluidic systems\; single extracellular vesicle (EV) size photometry &
  protein co-expression analysis using a conventional fluorescence microsco
 pe. Several companies were spun-off from his lab\, notably Sensoreal (capi
 llaric circuits) and Nomic Bio (affinity proteomics).\n\n\nZoom link (with
  one-time registration for the whole series) for attending remotely: https
 ://go.epfl.ch/EPFLBioETalks\n\n\nInstructions for 1st-year Ph.D. students 
 planning to attend this talk\, who are under EDBB’s mandatory seminar at
 tendance rule:\nIN CASE you cannot attend in-person in the room\, please m
 ake sure to\n\n	send D. Reinhard a note well ahead of time (ideally before
  seminar day)\, informing that you plan to attend the talk online\, and\, 
 during seminar:\n	be signed in on Zoom with a recognizable user name (not 
 any alias making it difficult or impossible to identify you).\n\nStudents 
 attending the seminar in-person should collect a confirmation signature af
 ter the talk - please print your own signature sheet beforehand (69 kB pdf
  available for download here). IMPORTANTLY: hang on to this sheet as no si
 gnature record is being kept by anyone else!
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://go.epfl.ch/
 EPFLBioETalks
STATUS:CONFIRMED
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