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SUMMARY:EPFL BioE Talks SERIES  "Structural and Molecular Basis of Nucleos
 ome Formation and Its Regulation"
DTSTART:20250331T121500
DTEND:20250331T131500
DTSTAMP:20260405T232259Z
UID:b5be88c27462eb9a5164c0e8e4003415bcd201482e339183c3a2b176
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Ji-Joon Song\, Department of Biological Sciences\, Korea
  Advanced Institute of Science and Technology (KAIST)\, Daejeon (KOR)\nWEE
 KLY EPFL BIOE TALKS SERIES (sandwiches provided)\n\nAbstract:\nNucleosome 
 assembly is essential for chromatin organization and gene regulation\, req
 uiring histone chaperones. Here\, we reveal the structural and molecular m
 echanisms of nucleosome formation and its regulation. We show that the ATA
 D2 homolog Abo1 facilitates ATP-dependent histone H3-H4 loading onto DNA\,
  as demonstrated by integrated biophysical approach using cryo-EM and sing
 le-molecule DNA curtain assay. Our further structural analysis reveals key
  conformational changes that enable histone deposition\, with high-speed a
 tomic force microscopy (HS-AFM) visualizing Abo1’s dynamic transitions. 
 In contrast\, nucleosome assembly is negatively regulated by CODANIN-1\, w
 hich inhibits the histone chaperone ASF1. Our cryo-EM structure of the COD
 ANIN-1_ASF1A complex reveals that CODANIN-1 sequesters ASF1 via B-domains 
 and histone mimic helices\, preventing H3-H4 complex formation and restric
 ting ASF1 in the cytoplasm. These findings provide structural insights int
 o the mechanisms of histone deposition and its regulation\, shedding light
  on the balance between nucleosome formation and inhibition.\n\nBio:\nJi-J
 oon Song is a Professor in the Department of Biological Sciences at KAIST 
 (Korea Advanced Institute of Science and Technology). He received his. B.S
 . at Seoul National University\, and his Ph.D. in structural biology at th
 e Cold Spring Harbor Laboratory. During his Ph.D.\, he determined the firs
 t structure of Argonaute and identified the Slicer in RNAi. He then joined
  Bob Kingston's group as a research fellow to investigate epigenetic mecha
 nism of gene regulation at Massachusetts General Hospital\, Harvard Medica
 l School. The research in Song Lab at KAIST focuses on understanding the m
 olecular mechanisms of epigenetic gene regulation and neurodegenerative di
 sease using integrative structural approaches.\n\n\nZoom link (with one-ti
 me registration for the whole series) for attending remotely: https://go.e
 pfl.ch/EPFLBioETalks\n\nInstructions for 1st-year Ph.D. students planning 
 to attend this talk\, who are under EDBB’s mandatory seminar attendance 
 rule:\nIN CASE you cannot attend in-person in the room\, please make sure 
 to\n\n	send D. Reinhard a note well ahead of time (ideally before seminar 
 day)\, informing that you plan to attend the talk online\, and\, during se
 minar:\n	be signed in on Zoom with a recognizable user name (not any alias
  making it difficult or impossible to identify you).\n\nStudents attending
  the seminar in-person should collect a confirmation signature after the t
 alk - please print your own signature sheet beforehand (69 kB pdf availabl
 e for download here). IMPORTANTLY: hang on to this sheet as no signature r
 ecord is being kept by anyone else!
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://go.epfl.ch/
 EPFLBioETalks
STATUS:CONFIRMED
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