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SUMMARY:BMI Distinguished Seminar // Kenneth Harris: Spike train structure
  of cortical transcriptomic populations in vivo
DTSTART:20251029T121500
DTEND:20251029T131500
DTSTAMP:20260317T100726Z
UID:af93f734f55b672dc4dea7270caa6ca8a175e706caa00f9c8dd77827
CATEGORIES:Conferences - Seminars
DESCRIPTION:Kenneth Harris\, UCL\, UK\nThe cortex comprises many neuronal 
 types\, which can be distinguished by their transcriptomes: the sets of ge
 nes they express.  Little is known about the in vivo activity of these ce
 ll types\, particularly as regards the structure of their spike trains\, w
 hich might provide clues to cortical circuit function. \n \nTo address t
 his question\, we used Neuropixels electrodes to record layer 5 excitatory
  populations in mouse V1\, then transcriptomically identified the recorded
  cell types.  To do so\, we performed a subsequent recording of the same 
 cells using 2-photon (2p) calcium imaging\, identifying neurons between th
 e two recording modalities by fingerprinting their responses to a “zebra
  noise” stimulus and estimating the path of the electrode through the 2p
  stack with a probabilistic method.  We then cut brain slices and perform
 ed in situ transcriptomics to localize ~300 genes using coppaFISH3d\, a ne
 w open source method\, and aligned the transcriptomic data to the 2p stack
 .  \n \nAnalysis of the data is ongoing\, and suggests substantial diff
 erences in spike time coordination between ET and IT neurons\, as well as 
 between transcriptomic subtypes of both these excitatory types.
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://epfl.zoom.u
 s/j/64813563657
STATUS:CONFIRMED
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