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PRODID:-//Memento EPFL//
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SUMMARY:Intracellular electrophysiology
DTSTART:20250909T161500
DTEND:20250909T180000
DTSTAMP:20260314T211159Z
UID:a1c4fca373a9afc759463ba2761f0695968cc65ee579a94cb180e2d6
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Yamuna Krishman (University of Chicago)\nSCS Lectureship
  Award 2025 : \nThe chemical milieu within an organelle has been evolutio
 narily optimized to enable the biochemistry that occurs within. We study h
 ow organelle function impacts cell function by mapping ions within the org
 anelle lumens using a chemical imaging technology based on DNA. DNA self-a
 ssembles into molecularly precise\, synthetic assemblies\, commonly referr
 ed to as DNA nanodevices. Our DNA nanodevices are ion responsive\, fluores
 cent probes that can be targeted to specific organelles(1). These reporter
 s can then quantitatively image ions in organelles of cells in culture\, i
 n live multicellular organisms (2) as well as in cells obtained from blood
  draws (3) or skin biopsies from human patients (4). I will focus on recen
 t findings from our lab. For example\, where we solved a thirty-year probl
 em in molecular sensing by mapping calcium in acidic organelles and identi
 fied the first example of a human lysosomal Ca2+ import mechanism (5)\, di
 scovered that organelles have electrical behavior (6)\, and more recently\
 , the discovery of sub-organellar pH gradients.\n\n====\n\nReferences:\n\n
 1.     Tinker\, J.\, et. al. Quantitative chemical imaging of organell
 es. Acc. Chem. Res.\, 2024\, 57\, 1906-1917.\n2.     Narayanaswamy\, N
 . et. al. A pH-correctable\, DNA-based fluorescent reporter for organellar
  Calcium. Nat. Methods\, 2019\, 16\, 95-102.\n3.     Cui\, C. et al. A 
 lysosome-targeted DNA nanodevice selectively targets macrophages to attenu
 ate tumours. Nat. Nanotechnol.\, 2021\, 16\, 1394-1402.\n4.     Leung\
 , K.\, et al. A DNA nanomachine chemically resolves lysosomes in live cell
 s. Nat. Nanotechnol.\, 2019\, 14\, 176-183.\n5.     Zajac M.\, et al. A
  mechanism of lysosomal calcium entry. Sci. Adv. 2024\, 10\, eadk2317.\n6.
      Saminathan A.\, et al. A DNA based voltmeter for organelles. Nat. 
 Nanotechnol. 2021\, 16. 96-103.\n\n 
LOCATION:CE 1 2 https://plan.epfl.ch/?room==CE%201%202
STATUS:CONFIRMED
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