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SUMMARY:Genome-wide in vitro reconstitution to study nucleosome positionin
 g and chromatin architecture
DTSTART:20260520T170000
DTEND:20260520T183000
DTSTAMP:20260511T050434Z
UID:91225e91911c7607240b3171c9e371cce8d1f6c586654f9c2b7a4ec9
CATEGORIES:Conferences - Seminars
DESCRIPTION:Elisa Oberbeckmann (University of Goettingen)\nAccess to genet
 ic information within the cell nucleus is regulated by the distribution of
  nucleosomes\, which are the basic unit of chromatin. Local access to spec
 ific genomic regions is facilitated by repositioning nucleosomes to enable
  transcription and other nuclear processes. Nucleosome positioning is prim
 arily regulated by ATP-dependent chromatin remodeling enzymes (CRs) that b
 elong to the Snf2-type helicase family. These enzymes disrupt histone-DNA 
 contacts by consuming ATP. The functions of CRs can be redundant or essent
 ial\, complicating their study in vivo. To address this\, we employ a uniq
 ue bottom-up approach\, in which we reconstitute chromatin in vitro using 
 purified proteins and a yeast genomic plasmid library. To elucidate the di
 verse remodeling functions of CRs\, we add purified CRs in combination wit
 h various transcription factors to the in vitro reconstituted chromatin. T
 he resulting changes in nucleosome positioning are monitored using MNase-
 seq. Depending on the type of CR used\, we observe distinct nucleosome pos
 itioning patterns. Furthermore\, we have expanded our in vitro reconstitut
 ion approach to explore the 3D genome organization of reconstituted chroma
 tin\, discovering a role for CRs in the 3D genome organization of S. cere
 visiae.
LOCATION:BCH 2218 https://plan.epfl.ch/?room==BCH%202218
STATUS:CONFIRMED
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