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SUMMARY:EPFL BioEngineering Talks
DTSTART:20261026T121500
DTEND:20261026T134500
DTSTAMP:20260925T034235Z
UID:2c7a9c60f7860f8938acdedbe4000c7b865c4c39f79c202dcf5f8632
CATEGORIES:Conferences - Seminars
DESCRIPTION:Invited Speaker and Student Speaker\nWeekly BioEngineering Tal
 ks (Lunch Provided)\n\nINVITED SPEAKER:\n\nAdvancing Multidimensional Sing
 le-Molecule localization microscopy: development and applications in nucle
 ar organization\nDr. Bassam Hajj\, Institut Curie\, Paris\nHost: Prof. Al
 eksandra Radenovic\n\nAbstract:\nSingle-molecule localization microscopy (
 SMLM) has revolutionized our understanding of biological processes by prov
 iding access to spatial scales beyond the diffraction limit of conventiona
 l optical microscopy. In the study of the cell nucleus\, SMLM has offered 
 unique insights into its organization and function at the nanoscale. Nucle
 ar architecture is structured across multiple spatial scales in three dime
 nsions and is highly dynamic. Understanding the mechanisms that govern nuc
 lear organization therefore requires imaging approaches capable of capturi
 ng molecular dynamics across a broad range of spatial and temporal scales 
 and in diverse biological contexts. Although SMLM can routinely achieve sp
 atial resolutions in the tens-of-nanometers range\, many biologically rele
 vant structures and processes remain beyond its reach. For example\, resol
 ving the precise organization of DNA at the molecular scale remains challe
 nging\, as it would require a substantial improvement in spatial resolutio
 n over conventional SMLM\, while also addressing the high density and degr
 ee of molecular compaction within the nucleus. \nOver the years\, we have
  developed complementary strategies to address these challenges by introdu
 cing additional dimensions and observables into SMLM measurements. In this
  presentation\, I will first discuss our approaches to three-dimensional s
 ingle-molecule imaging\, including multifocus microscopy and volumetric li
 ght-sheet excitation\, which enable fast and sensitive imaging over extend
 ed volumes and in complex biological samples such as cell colonies. While 
 these approaches provide access to the three-dimensional and temporal dime
 nsions required to investigate nuclear organization\, spatial resolution a
 lone is not always sufficient to distinguish molecular arrangements. We ha
 ve therefore extended SMLM further by implementing polarization-resolved m
 ultifocus microscopy (PolMFM)\, which combines three-dimensional localizat
 ion with information on molecular orientation.\nI will illustrate how thes
 e multidimensional approaches can provide new insights into nuclear organi
 zation\, with examples spanning different biological systems. In particula
 r\, I will present evidence for a long-range\, crystal-like organization o
 f DNA within the nuclei of mature sperm cells from crickets\, demonstratin
 g how multidimensional imaging can reveal structural features that remain 
 inaccessible to conventional localization microscopy.\nFinally\, I will di
 scuss the challenges and opportunities associated with visualizing\, analy
 zing\, and interactively exploring the multidimensional datasets generated
  by these approaches. Together\, these developments illustrate how extendi
 ng SMLM beyond conventional two-dimensional localization can provide new w
 ays to interrogate the organization and dynamics of biological systems at 
 the nanoscale.\n\n\nBio:\nBassam Hajj is a physicist and CNRS researcher a
 t Institut Curie in Paris\, where he is a member of the Physics of Cells a
 nd Cancer unit and co-leads the “Light-based Observation and Control of 
 Cellular Organization” team. His research focuses on the development of 
 advanced optical microscopy techniques\, particularly single-molecule loca
 lization microscopy\, 3D imaging\, and multidimensional approaches for stu
 dying cellular organization at the nanoscale.\nAfter completing his PhD at
  École Normale Supérieure de Cachan on electro-optical microscopy\, Bass
 am joined Janelia Research Campus as a postdoctoral researcher\, where he 
 worked on multifocus microscopy for fast and sensitive single-molecule ima
 ging. He subsequently returned to Institut Curie and joined the CNRS as a 
 permanent researcher in 2016.\nHis current research combines innovative mi
 croscopy\, quantitative image analysis\, and data visualization to investi
 gate molecular organization and dynamics\, with a particular focus on nucl
 ear organization.\n\nSTUDENT SPEAKER:\n\nTranscription Factor Dose in Stem
  Cell Reprogramming: Insights from Single-Cell Multi-Omics\nAngelika Gebha
 rt\, Deplancke Lab\n\n\n-------------------------------------------------
 -----------------------------\n\nBioEngineering Talks mandatory EDBB Semin
 ar Attendance (1st-Year PhD Students)\n\n	 Attendance sheet to print HERE
  \n\nThe BioEngineering Student Seminar Series is an official course for 
 which students can register and earn credits.\n \n\n	Register HERE\n	Atte
 ndance sheet to print HERE\n\nGeneral information:\nIn-person attendance i
 s preferred to support your fellow students. Zoom is mainly for students o
 n remote campuses\; please notify Fiorella Ghisays in advance and join usi
 ng your full name.\nIf attending in person\, have your sheet signed after 
 the talk and keep the original\, as no copy is retained.\n 
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717 https://epfl.zoom.u
 s/j/68746410793
STATUS:CONFIRMED
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