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SUMMARY:Enzyme Proximity Sequencing: Dissecting the Complexity of Enzymes 
 at Scale - CH-636
DTSTART:20261124T161500
DTEND:20261124T171500
DTSTAMP:20261109T173509Z
UID:93501069100c55235e9a28877d479225812016d10cfb23bf3088f332
CATEGORIES:Conferences - Seminars
DESCRIPTION:Rosario Vanella\, Senior Scientist at the Department of Chemis
 try\, University of Basel\nSpeaker: Rosario Vanella (Senior Scientist at 
 the Department of Chemistry\, University of Basel)\n\nAbstract: Enzymes ar
 e complex molecular systems in which a single amino acid sequence and thre
 e-dimensional structure encode multiple interconnected properties. Individ
 ual mutations can therefore affect several dimensions of enzyme fitness\, 
 including folding stability\, catalytic activity and substrate specificity
 . Understanding these effects and their relationships is essential for eng
 ineering enzymes for applications in industry and biomedicine. However\, s
 ystematically mapping how sequence changes reshape these properties at sca
 le remains challenging for many enzyme classes. A key difficulty is measur
 ing enzyme activity at high throughput while maintaining a reliable link b
 etween each variant’s sequence and its function. This seminar will prese
 nt the development and applications of Enzyme Proximity Sequencing (EP-Seq
 )\, a yeast-based platform that combines enzyme surface display with proxi
 mity-based\, activity-dependent cell labeling. EP-Seq enables thousands of
  enzyme variants to be profiled across multiple functional properties and 
 substrates. The resulting datasets help elucidate sequence-structure-funct
 ion relationships\, identify beneficial mutations and guide enzyme enginee
 ring through both experimental and AI-guided strategies.\n\nBiography: Ro
 sario Vanella studied biotechnology at the University of Palermo (Italy). 
 He conducted his doctoral research at FHNW in collaboration with the biote
 chnology company Evolva\, receiving his Ph.D. in biochemistry from the Bio
 zentrum\, University of Basel\, in 2015.  In 2016\, he joined Michael Nas
 h’s group at the University of Basel and ETH Zurich (D-BSSE) as a postdo
 ctoral fellow. Since 2022\, he has been a Senior Scientist in the same gro
 up. His research combines high-throughput methods and protein engineering 
 to develop proteins for industrial and therapeutic applications. He develo
 ped Enzyme Proximity Sequencing (EP-Seq)\, a platform for profiling thousa
 nds of enzyme variants across multiple functional properties and substrate
 s.\n 
LOCATION:CE 1 105 https://plan.epfl.ch/?room==CE%201%20105
STATUS:CONFIRMED
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