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SUMMARY:What do microparticles from blood plasma look like? Their morpholo
 gy\, size and phenotype revealed by cryo-EM and immuno-gold labeling 
DTSTART:20130321T110000
DTEND:20130321T120000
DTSTAMP:20260510T032014Z
UID:f0b353bb9a0dd69df1e7cbd338b4fbd62f6c832eb584a889e2c7e60a
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Alain Brisson\, Université Bordeaux\nMicroparticles (MP
 s)\, also called extracellular vesicles or microvesicles\, are cell membra
 ne\nfragments derived from activated or apoptotic cells. MPs attract high 
 interest as potential disease\nbiomarkers because they contain elements\, 
 like surface receptors\, specific from their parental cells.\nHowever many
  basic questions concerning MPs remain unanswered\, such as: What do MPs f
 rom\nblood plasma look like? What is their morphology\, their size distrib
 ution? Do they all bind Annexin-5\n(Anx5)? How are they formed? How many a
 re they?\nOur overall aim is to answer these questions\, firstly to improv
 e our basic understanding on MPs\,\nsecondly to design diagnosis assays on
  selected MP sub-populations. Our first objective was to\nprovide a catalo
 gue of all types of MPs present in plasma from healthy donors\, considerin
 g this as a\nreference for further studies of MPs in various pathological 
 conditions.\nWe have developed an original approach based on cryo-Transmis
 sion Electron Microscopy\n(cryo-EM) imaging and receptor-specific gold lab
 eling. Cryo-EM allows revealing the\nvarious types of MPs present in pure 
 platelet free plasma (PFP). Gold nanoparticles\nfunctionalized with variou
 s ligands were developed for identifying specific MP subpopulations.\nWe f
 ocused our attention primarily on MPs exposing phosphatidylserine (PS)\,\n
 MPs derived from platelets and MPs derived from red blood cells\, using go
 ld particles\nfunctionalized with Anx5\, anti-CD41 and anti-glycophorin an
 tibodies\, respectively
LOCATION:CH G1 495 https://plan.epfl.ch/?room==CH%20G1%20495
STATUS:CONFIRMED
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