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SUMMARY:Advances in Imaging Fluorescence Correlations Spectroscopy
DTSTART:20130902T160000
DTEND:20130902T170000
DTSTAMP:20260407T074524Z
UID:79dc0e4b7c386e5c0b79d3629334448a10a80fc74b6ed3accc74fa3c
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Thorsten WOHLAND\, Dept of Biological Sciences and Chemi
 stry\, National University of Singapore\nImaging Fluorescence Correlation 
 Spectroscopy (Imaging FCS) has emerged as a powerful technique to multiple
 x FCS and allow the recording of FCS images. This provides a number of dis
 tinct advantages: 1) One can collect thousands of correlation functions si
 multaneously resulting in excellent statistics. 2) The imaging format cont
 ains spatio-temporal information which provides insights into structure an
 d dynamics of the sample. In particular the FCS diffusion laws\, which can
  provide information of the structure of a sample beyond the optical resol
 ution limit\, are simple to implement within Imaging FCS. 3) Light sheet b
 ased illumination schemes require a much lower light exposure per measurem
 ent and thus allow more measurements to be collected per sample. 4) The im
 aging mode in time allows the recording of FCS time lapse videos to observ
 e changes in molecular dynamics over time. Here we demonstrate the capabil
 ities of imaging FCS in 2D and 3D using total internal reflection (TIR) an
 d single plane illumination microscopy (SPIM)\, as illumination modes\, re
 spectively. We investigate the membrane active human Islet Amyloid Polypep
 tide (hIAPP) on cell membranes using imaging TIR-FCS\, and investigate mol
 ecular interactions in 3D by SPIM-FCS and FCCS. These measurements demonst
 rate that Imaging FCS can provide quantitative images of biological sample
 s
LOCATION:CH B3 30
STATUS:CONFIRMED
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