BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Memento EPFL//
BEGIN:VEVENT
SUMMARY:Mitochondrial biogenesis in the rodent CNS – an in vivo study
DTSTART:20140217T103000
DTEND:20140217T113000
DTSTAMP:20260427T221139Z
UID:215ef0bd8ed632fc909e3fce5ce0285fb472b44d0aff639fdc5e7852
CATEGORIES:Conferences - Seminars
DESCRIPTION:Radha DESAI\nUniversity of London (UCL)\, London\, UK\nReplica
 tion of mitochondrial DNA is an essential component of growth of the mitoc
 hondrial network or biogenesis. We optimized a technique\, which tags repl
 icating mitochondrial DNA using a thymidine analogue\, bromodeoxyuridine (
 BrdU) to investigate the changes in this biogenesis signal in rodent model
 s of neuroinflammation. Upon scanning of the CNS using the BrdU method\, w
 e found that there are certain hotspots of mtDNA replication in neuronal p
 opulations\, which implicates a stark difference in the rate of mitochondr
 ial turnover. It turns out that these cells correspond to regions of the b
 rain\, which are known to be vulnerable in certain diseases with a mitocho
 ndrial component to pathology.\nA time series investigation of a ‘pulse 
 and chase’ protocol also suggested that while mitochondria are distribut
 ed all through motor neurons\, the DNA replication signal only appears in 
 the cell body initially and then slowly distributes to the extremities of 
 the axons. Furthermore\, the signal we observe responds to changes in ener
 gy demand\, as tested by unilateral electrical stimulation of the sciatic 
 nerve. This suggests an increase in mitochondrial biogenesis in correspond
 ing motor neuron cell bodies as well as dorsal root ganglion cells. With t
 he generation of new drugs\, which target the mitochondrial life cycle\, t
 his work provides critical information\, which can aid the targeting of mi
 tochondrial dynamics as a therapeutic target in neurological disease.
LOCATION:AI 1153 https://plan.epfl.ch/?room==AI%201153
STATUS:CONFIRMED
END:VEVENT
END:VCALENDAR