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SUMMARY:Mitochondrial biogenesis in the rodent CNS – an in vivo study
DTSTART:20140217T103000
DTSTAMP:20260428T094020Z
UID:43819a024fb741db5ba6b0cffefb37d365db486303f7f1853e3fba41
CATEGORIES:Conferences - Seminars
DESCRIPTION:Radha Desai\, University College London (UCL)\, London\, UK\nD
 etails: see http://uk.linkedin.com/pub/radha-desai/26/370/268\nA seminar o
 f the Lausanne Integrative Metabolism and Nutrition Alliance (LIMNA)\nAbst
 ract:\nReplication of mitochondrial DNA is an essential component of growt
 h of the mitochondrial network or biogenesis. We optimized a technique\, w
 hich tags replicating mitochondrial DNA using a thymidine analogue\, bromo
 deoxyuridine (BrdU) to investigate the changes in this biogenesis signal i
 n rodent models of neuroinflammation. Upon scanning of the CNS using the B
 rdU method\, we found that there are certain hotspots of mtDNA replication
  in neuronal populations\, which implicates a stark difference in the rate
  of mitochondrial turnover. It turns out that these cells correspond to re
 gions of the brain\, which are known to be vulnerable in certain diseases 
 with a mitochondrial component to pathology.\nA time series investigation 
 of a ‘pulse and chase’ protocol also suggested that while mitochondria
  are distributed all through motor neurons\, the DNA replication signal on
 ly appears in the cell body initially and then slowly distributes to the e
 xtremities of the axons. Furthermore\, the signal we observe responds to c
 hanges in energy demand\, as tested by unilateral electrical stimulation o
 f the sciatic nerve. This suggests an increase in mitochondrial biogenesis
  in corresponding motor neuron cell bodies as well as dorsal root ganglion
  cells. With the generation of new drugs\, which target the mitochondrial 
 life cycle\, this work provides critical information\, which can aid the t
 argeting of mitochondrial dynamics as a therapeutic target in neurological
  disease.
LOCATION:AI 1 153 http://map.epfl.ch/?room=ai1153
STATUS:CONFIRMED
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