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SUMMARY:Microfabricated bioreactor devices for bioprocess development
DTSTART:20140131T130000
DTEND:20140131T140000
DTSTAMP:20260510T164923Z
UID:e7be386a2e3323afddf3d3aea292b4a871acc6fd927c012113062b63
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr Nicolas Szita\nUniversity College London\, Department of Bi
 ochemical Engineering\, United Kingdom\nABSTRACT :\nOver the last ten year
 s\, bioreactor miniaturisation has made significant progress in traditiona
 l biotechnology and has changed the way early stage process development ca
 n be approached. Key advantages that make microfabricated bioreactors a co
 st-effective proposition for early bioprocess development include: signifi
 cant reduction in reagent use\, real-time monitoring and control of proces
 s variables\, ease of sterilisation via disposable polymer technology\, re
 duced labour due to automation\, and the capability to rapidly test differ
 ent processing conditions. In my presentation\, I will highlight these adv
 ances and demonstrate how microfabricated bioreactors will make an impact 
 in emerging areas for bioprocessing.\nFor cell therapy\, the development o
 f tightly controlled culture processes will be crucial for the clinical an
 d commercial use of pluripotent cells\, and novel tools are direly needed.
  To address this\, we have developed a unique microfabricated cell culture
  device (‘micro bioreactor’) which uses microfluidic approaches to con
 trol the microenvironment of the cells\, and which maintains a link with t
 raditional small-scale culture devices for validation and scale-up studies
 . Analytical methods are integrated with the device to monitor cell cultur
 e growth online\, generating robust quantitative data suitable for process
  documentation or evaluation of experimental outcomes. We have tested the 
 device using human and mouse embryonic stem cell expansion protocols as a 
 model system.\nIn synthetic biology\, the key challenge will be to achieve
  manufacturability of the many engineered cells that this emerging field e
 ndeavours to produce. In my group\, a microfabricated bioreactor was devel
 oped which allows controlled exposure of de novo synthesised expression el
 ements to defined growth conditions. This so-called micro-chemostat contai
 ns a novel electro-magnetic stirrer\, real-time monitoring of growth condi
 tions and fluorescent gene product expression\, and a demonstrator multipl
 exed system has been established. In ongoing work\, the capability of this
  micro-chemostat for rapid acquisition of design-relevant gene expression 
 parameters in statistical depth is explored for the gram-positive S. carno
 sus as a chassis for lantibiotic producing genes within the framework of a
  European project.A
LOCATION:CM 1 4 https://plan.epfl.ch/?room==CM%201%204
STATUS:CONFIRMED
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