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SUMMARY:Sensing Single Biomolecules using Protein and Solid-State Nanopore
 s
DTSTART:20140313T101500
DTSTAMP:20260413T105825Z
UID:90a948defe42618dea592cebb632b784951b342f3e8aa57c71032f11
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Amit Meller\, The Technion – Israel Institute of Techn
 ology\, Haifa (IL) and Boston University\, Boston\, MA (USA)\nBio: 2010- 
  Professor of Biomedical Engineering\, Technion - Israel Institute of Tech
 nology\, Israel\n2010-  World-Class University visiting Professor\, Depar
 tment of Biophysics and Chemical Biology\, Seoul National University\, Seo
 ul\, S. Korea\n2006-  Associate Professor of Biomedical Engineering\, Ass
 ociate Professor of Physics\, Boston University\n2000 - 2006  Rowland Sen
 ior Fellow\, PI of the Single Molecule Biophysics lab\, Rowland Institute\
 , Harvard University\n1998 - 2000  Postdoc Fellow\, Department of Molecul
 ar and Cellular Biology\, Harvard University with Prof. Dan Branton\n1997 
 - 1998  Postdoc Fellow\, Department of Molecular Genetics\, Weizmann Inst
 itute of Science with Prof. Doron Lancet\n1997  Ph.D. Physics\, Weizmann 
 Institute of Science\n1993  M.Sc. Physics\, Weizmann Institute of Science
 \n1989  B.Sc. Physics and Astronomy\, Tel Aviv University\nBIOENGINEERING
  SEMINARAbstract:\nNanopores can be used to detect and characterize unlabe
 led biomolecules\, and widely believed to be a main future platform for di
 rect\, single molecule sequencing of DNA\, RNA and perhaps proteins.(1) Co
 ntrolling and tuning the capture rate and the translocation speed of biomo
 lecules through nanopores remains to be a main challenge for this technolo
 gy\, limiting their wider application. In this lecture I will discuss two 
 physical methods to: (i) enhance the capture rate of DNA molecules into so
 lid-state using salt gradients thus enabling ultra sensitive sensing down 
 to attomoles (2)\, and (ii) slowing down the translocation speed of DNA an
 d proteins through nanopores using a novel optoelectronic effect that can 
 be switched on/off in a fraction of a millisecond.(3)\nNanpores can also b
 e used to detect and map DNA and RNA-protein interactions. I will discuss 
 single molecule nanopore measurements of Poly Adenine Binding Proteins (PA
 BPs) associated with translation regulation with poly-Adenine RNAs(4)\, as
  well as the interactions of transcription factors with genomic DNA\n(1). 
 Wanunu\, M. & Meller\, A. in Laboratory Manual on Single Molecules Vol. 39
 5-420 (eds T. Ha & P. Selvin)  (Cold Spring Harbor Press\, 2008).\n(2). W
 anunu\, M.\, Morrison\, W.\, Rabin\, Y.\, Grosberg\, A. Y. & Meller\, A. E
 lectrostatic Focusing of Unlabeled DNA into Nanoscale Pores using a Salt G
 radient. Nature Nanotechnology  5\, 160-165 (2010).\n(3). Di Fiori\, N.\,
  Squires\, A.\, Bar\, D.\, Gilboa\, T.\, Moustakas\, T. and A. Meller.  O
 ptoelectronic control of surface charge and translocation dynamics in soli
 d-state nanopores. Nature Nanotechnology 8\, 946–951 (2013).\n(4). Lin\,
  J.\, Fabian\, M.\, Sonenberg\, N. and A. Meller\, Nanopore Detachment Kin
 etics of Poly(A) Binding Proteins from RNA Molecules Reveals the Critical 
 Role of C-Terminus Interactions. Biophysical Journal 102\, 1427–1434 (20
 12).
LOCATION:SV1717A http://map.epfl.ch/?room=sv1717a
STATUS:CONFIRMED
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