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SUMMARY:"Signal Transduction during Axon Guidance Investigated with Image 
 Cross-Correlation Spectroscopy and FRET"
DTSTART:20101217T121500
DTSTAMP:20260921T194907Z
UID:755b877c5234657f835d9206f407324f21085fcc6cde8ca8eaf4bff8
CATEGORIES:Conferences - Seminars
DESCRIPTION:Benjamin Rappaz\, McGill University\, Montréal\, Canada\nDuri
 ng central nervous system development neurons extend axons over relatively
  great distance to reach their ultimate synaptic targets. Netrin is one of
  the main axon guidance cues whose effect is mediated by its two receptors
  Unc5 and DCC. DCC alone is known to promote attraction\, whereas DCC in c
 ombination with Unc5 promotes repulsion.\nMy current project proposes to i
 nvestigate the molecular interactions between Netrin and its receptors\, a
 nd part of the downstream signaling mechanisms involved using state of the
  art optical imaging techniques in living neurons.\nImage Cross-Correlatio
 n Spectroscopy (ICCS) and Fluorescence Resonance Energy Transfer (FRET) ar
 e two complementary imaging techniques which measure co-localization of fl
 uorescently tagged proteins.\nIn this presentation\, I will introduce the 
 ICCS theory and present Unc5/DCC colocalisation data I obtained with this 
 technique. FRET was then used to measure the spatio-temporal activation of
  Cdc42\, a small Rho GTPase activated downstream of DCC which promotes fil
 ipodia growth.
LOCATION:AI1153
STATUS:CONFIRMED
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