BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Memento EPFL//
BEGIN:VEVENT
SUMMARY:A Single Peptide-MHC Triggers Digital Cytokine Secretion in CD4+ T
  Cells
DTSTART:20140401T143000
DTSTAMP:20260916T002741Z
UID:5adb2ecfc9f28fb7d3355e51db9025b4b2e73ecc5179ea875ce222e1
CATEGORIES:Conferences - Seminars
DESCRIPTION:Jun Huang\, Ph.D.\, Stanford University\, Stanford\, CA (USA)\
 nBIOENGINEERING SEMINARAbstract:\nWe have developed a single-molecule imag
 ing technique that uses quantum-dot-labeled peptide-major histocompatibili
 ty complex (pMHC) ligands to study CD4+ T cell functional sensitivity. We 
 found that naive T cells\, T cell blasts\, and memory T cells could all be
  triggered by a single pMHC to secrete tumor necrosis factor alpha (TNF-a)
  and interleukin-2 (IL-2) cytokines with a rate of ~1\,000\, ~10\,000\, an
 d ~10\,000 molecules/min\, respectively\, and that additional pMHCs did no
 t augment secretion\, indicating a digital response pattern. We also found
  that a single pMHC localized to the immunological synapse induced the slo
 w formation of a long-lasting T cell receptor (TCR) cluster\, consistent w
 ith a serial engagement mechanism. These data show that scaling up CD4+ T 
 cell cytokine responses involves increasingly efficient T cell recruitment
  rather than greater cytokine production per cell.Bio:\n2008 Ph.D.\, Bioen
 gineering\, Georgia Institute of Technology\, Atlanta\, GA\, USA (advisor:
  Professor Cheng Zhu)\n2007 M.S.\, Chemical Engineering\, Georgia Institut
 e of Technology\, Atlanta\, GA (USA)\n2003 M.S.\, Biomedical Engineering\,
  Institute of Mechanics\, Chinese Academy of Sciences and Chongqing Univer
 sity (Joint Program)\, Beijing\, China\n2000 B.S. (with honor)\, Chemical 
 Engineering\, Chongqing University\, Chongqing\, China\nPostdoctoral train
 ing:\n2009–     Department of Microbiology and Immunology\, Howard Hug
 hes Medical Institute\, Stanford University\, Stanford\, CA\, USA (advisor
 : Professor Mark M. Davis)
LOCATION:SV1717A http://map.epfl.ch/?room=sv1717a
STATUS:CONFIRMED
END:VEVENT
END:VCALENDAR