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SUMMARY:Single-Cell RNA-seq Reveals Principles of Allelic Expression in Ma
 mmalian Cells
DTSTART:20140501T110000
DTSTAMP:20260429T081254Z
UID:359ccc53f5ddc9fd9358349d3d73ae67e64fef8a5270305af7c88ae1
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Rickard Sandberg\, Karolinska Institutet and Ludwig Inst
 itute for Cancer Research\, Stockholm (SE)\nBIOENGINEERING SEMINARAbstract
 :\nAssessing gene expression in single cells will be critical to better un
 derstand cellular behaviors and compositions in developing\, adult and pat
 hological tissues. To this end\, my lab has been developing single-cell RN
 A-seq methods (e.g. Smart-seq2) that combine full-length transcript covera
 ge with high sensitivity and accuracy. We have performed global analyses o
 f gene expression in hundreds of individual cells from mouse preimplantati
 on embryos of mixed genetic background (CAST/EiJ x C57BL/6J). Using strain
 -specific SNPs we investigate allelic expression in these cells during pre
 implantation development to characterize X-chromosome imprinting and allel
 ic expression patterns of autosomal genes. Although expression from both p
 aternal alleles is generally observed in analyses of diploid cell populati
 ons\, no study has addressed allelic expression patterns genome-wide in si
 ngle cells. Importantly\, we demonstrate that allelic expression is indepe
 ndent for genes across all expression levels making this a genomic princip
 le. Additionally\, our study revealed that allelic transcription fluctuati
 ons generated widespread random monoallelic expression in single cells\, a
 nd we discuss its functional consequences.Bio:\n2009 - Present   Member 
 at Ludwig Institute for Cancer Research\, Stockholm (SE)\n2007 - present 
   Professor (Assistant\, then Associate)\, Karolinska Institutet\, Stockh
 olm (SE)\nEducation:\n2004 - 2006   Postdoctoral Fellow\, MIT\, Cambridg
 e\, MA (USA)\n2003   PhD\, Karolinska Institutet\, Stockholm (SE)
LOCATION:SV1717A http://map.epfl.ch/?room=sv1717a
STATUS:CONFIRMED
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