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SUMMARY:Light-Synchronized Transcription Dynamics Indicates cis-Regulation
  of Transcription at Refractory Promoters
DTSTART:20140715T111500
DTSTAMP:20260407T103231Z
UID:f423f14e27f03953f720cac745fce40d33de204b3880754efad3abb7
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Michael Brunner\, Biochemistry Center\, Heidelberg Unive
 rsity (D)\nBIOENGINEERING SEMINARAbstract:\nTranscription of genes appears
  to be a smooth process when analyzed in an ensemble of cells. However\, o
 n the level of a cell many genes appear to be transcribed in random bursts
  followed by periods during which promoters are refractory towards re-stim
 ulation. We used the light-dependent transcription factor White Collar Com
 plex of Neurospora crassa as an optogenetic tool to study transcriptional 
 bursting in a population approach. Activation of the WCC by a light pulse 
 triggers a synchronized wave of transcription from the frequency promoter 
 followed by an extended refractory period of more than 1 h. When challenge
 d by a second light pulse\, stable binding of Pol II and phosphorylation o
 f its C-terminal domain repeats at serine 5 is impaired. We present eviden
 ce that refractory promoters carry a physical memory of their previous his
 tory.Bio:\nMichael Brunner is the director of the Heidelberg University Bi
 ochemistry Center and full professor since 2000 leading the "Circadian Rhy
 thms and Molecular Clocks" group. Their aim is to understand how the circa
 dian clock works as a program that coordinates complex expression profiles
  in a temporal fashion\, using the filamentous fungus Neurospora crassa as
  a model organism. The mechanisms underlying the positive and negative fee
 dback loops of the molecular clock and the temporal and spatial coordinati
 on of the various clock protein interactions are in the main focus of the 
 research. The FRQ/WCC oscillator modulates rhythmic expression of about 10
 00 Neurospora genes. Only a fraction of these genes are directly controlle
 d by WCC. The organization and regulation of the network of clock-controll
 ed genes is a further focus of the lab.
LOCATION:SV1717a http://map.epfl.ch/?room=sv1717a
STATUS:CONFIRMED
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