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PRODID:-//Memento EPFL//
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SUMMARY:Mapping regulatory genetic interaction in membrane trafficking
DTSTART:20150212T131500
DTEND:20150212T141500
DTSTAMP:20260509T054228Z
UID:5de24601eeb5a3a50ba8a5ada447ab5396571526f5ab99a8563459af
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr. Prisca Liberali\nInstitute of Molecular Life Sciences\,\nU
 niversity of Zürich\nEndocytosis is critical for cellular physiology and 
 thus highly regulated. To identify regulatory interactions controlling the
  endocytic membrane system\, we conducted 13 RNAi screens on multiple endo
 cytic activities and their downstream organelles. Combined with image anal
 ysis of thousands of single cells per perturbation and their cell-to-cell 
 variability\, this created a high-quality and cross-comparable quantitativ
 e dataset. Unbiased analysis revealed novel emergent properties of the end
 ocytic membrane system and how its complexity evolved\, and distinct progr
 ams of regulatory control that co-regulate specific subsets of endocytic u
 ptake routes and organelle abundances. We show that these subset effects a
 llow the mapping of functional regulatory interactions and their interacti
 on motifs between kinases\, membrane trafficking machinery\, and the cytos
 keleton at a large scale\, some of which we further characterise. This wor
 k presents a powerful approach to identify regulatory interactions in comp
 lex cellular systems from parallel single-gene or double-gene perturbation
  screens in human cells and yeast. Unpublished data on the molecular basis
  of the cell-to-cell variability determined by the cell cycle and the micr
 oenvironment will also be presented.
LOCATION:SV 1717a http://plan.epfl.ch/?zoom=20&recenter_y=5864073.98412&re
 center_x=730632.15084&layerNodes=fonds\,batiments\,labels\,information\,pa
 rkings_publics\,arrets_metro\,transports_publics&floor=1&q=sv_1717a
STATUS:CONFIRMED
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