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VERSION:2.0
PRODID:-//Memento EPFL//
BEGIN:VEVENT
SUMMARY:New frontiers in super-resolution biological imaging  and optogene
 tic probing of neuronal circuits
DTSTART:20100505T133000
DTSTAMP:20261002T014522Z
UID:8a2d032b005ce5928f76719abf1b247680881647f7b65ac269d729a0
CATEGORIES:Conferences - Seminars
DESCRIPTION:Alipasha Vaziri - Janelia Farm Research Campus\,  Howard Hughe
 s Medical Institute\nRecent advances in optical microscopy have enabled im
 aging of biological samples beyond the diffraction limit at nanometer reso
 lution. A common shortcoming has been that the imaging depth was limited t
 o a fraction of an optical wavelength. Yet\, to study whole cells\, the ex
 tension of these methods to a 3D-super-resolution technique at depth is re
 quired. I will present how we have overcome this limitation by utilizing t
 he two-photon excitation technique of temporal focusing in which the spect
 ral properties of ultrafast optical pulses are used to control the axial i
 ntensity distribution in space. Using this technique we have demonstrated 
 multilayer super-resolution imaging at ~50nm lateral resolution over an ax
 ial range of ~10μm in various biological samples.
LOCATION:SV1717a
STATUS:CONFIRMED
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