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SUMMARY:Nanoparticle-cell interactions: Importance of protein structure
DTSTART:20150828T140000
DTSTAMP:20260509T191250Z
UID:d31ed28d93636f88bb8979d1d73f177a7a5ae046dbb96d19e8d25822
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Christine Payne\, Georgia Institute of Technology\nNanop
 articles offer exciting new approaches for biomedicine ranging from drug d
 elivery to cellular imaging. In the course of these applications\, nanopar
 ticles are exposed to a complex mixture of extracellular proteins that ads
 orb onto the surface of the nanoparticle. This “protein corona” domina
 tes the interaction of nanoparticles with cells.\nWe have investigated how
  proteins found in blood serum affect the cellular binding of protein-nano
 particle complexes. Using fluorescence microscopy\, we find that the cellu
 lar binding of cationic nanoparticles is enhanced by the presence of serum
  proteins while the binding of anionic nanoparticles is inhibited. Competi
 tion assays confirm that these protein-nanoparticle complexes use distinct
  cellular receptors. Circular dichroism spectroscopy\, fluorescence spectr
 oscopy\, and isothermal titration calorimetry show that the secondary stru
 cture of the adsorbed serum albumin is altered following adsorption on the
  surface of cationic nanoparticles. These structural changes redirect the 
 albumin-nanoparticle complex to scavenger receptors. In comparison\, the s
 econdary structure of albumin is preserved following adsorption on anionic
  nanoparticles.\nThe cellular binding trend is independent of nanoparticle
  composition: quantum dots\, colloidal gold nanoparticles\, and low-densit
 y lipoprotein particles all show the same behavior. This link between prot
 ein structure and cellular outcomes will provide a molecular basis for the
  design of nanoparticles for use in biomedical applications.\nBio: Educati
 onal Background\n1998 B.S.\nChemistry\nUniversity of Chicago\nAdvisors: Ja
 mes Norris\, Norbert Scherer\n2003 Ph. D. Chemistry\nUniversity of Califor
 nia\, Berkeley\nAdvisor: Charles Harris\nEmployment History\n2007-\nAssist
 ant Professor\, Georgia Institute of Technology\n2003-2006\nPostdoctoral F
 ellow\, Harvard University\, Advisor: Xiaowei Zhuang\n1998-2000\nGraduate 
 Teaching Assistant\, University of California\, Berkeley
LOCATION:MXG 110 http://map.epfl.ch/?room=mxg110
STATUS:CONFIRMED
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