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SUMMARY:Genome Editing Comes of Age – Lessons learned from high throughp
 ut CRISPR targeting in human cell lines
DTSTART:20151002T130000
DTEND:20151002T140000
DTSTAMP:20260506T110801Z
UID:ee19d771492babbd98b7a231d9895e83ae8483f60e3c76315b7f9698
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr Chris Thorne\, Commercial Marketing Manager\nHorizon Discov
 ery Ltd\nCambridge\, CB25 9TL\, United Kingdom\nEmail: C.Thorne@horizondis
 covery.com   Website: www.horizondiscovery.com\nIn just a short period o
 f time CRISPR-Cas9 technology has revolutionized the field of genome editi
 ng\, and taken the scientific community by storm. Already our understandin
 g of how best to apply this technology has advanced significantly and almo
 st every week new publications appear showcasing its application in basic 
 and translational research.\nWhile CRISPR-Cas9 is applicable across many d
 ifferent cell types\, we have found it particularly suited for genome edit
 ing in near-haploid human cell lines. This has allowed us to establish a r
 obust pipeline for the inactivation of non-essential genes at unprecedente
 d scale and efficiency.\nWe have now knocked out over 1500 human genes and
  have generated a resource that is\, to the best of our knowledge\, the la
 rgest collection of human knockout cell lines available\, covering compreh
 ensive subsets of genes clustered by biological pathway (e.g. the autophag
 y pathway\, the JAK/STAT pathway) or by phylogenetic relationship (e.g. ki
 nases\, bromodomain-containing proteins).\nIn this talk we will discuss ho
 w\, through more than 1500 genome editing experiments\, we have started to
  unravel some of the general principles governing the use of CRISPR-Cas9 i
 n mammalian cells. For example\, we have analyzed the impact of variation 
 in the guide RNA sequence on Cas9 cleavage efficiency and characterized th
 e mutational signature arising from CRISPR-Cas9 cleavage.\nWe will also hi
 ghlight (with examples) how these learnings are now being applied to intro
 duce other genomic modifications in a high throughput manner\, including c
 hromosomal deletions\, translocations\, point mutations and endogenous gen
 e tags.
LOCATION:AI 1153 https://plan.epfl.ch/?room==AI%201153
STATUS:CONFIRMED
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