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SUMMARY:A Journey Through Protein Mass Spectrometry: From Computer Simulat
 ions of the Electrospray Process to Functional Studies on Molecular Motors
DTSTART:20160407T163000
DTEND:20160407T173000
DTSTAMP:20260922T014126Z
UID:5f70c0e3ffe5be17afe7f0b75ade2b302ced7c77fa68a0454e5b4a0e
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Lars Konermann\nProtein Mass Spectrometry Laboratory\, 
 Department of Chemistry / Department of Biochemistry\, The University of 
 Western Ontario\, London\, Canada\nElectrospray ionization (ESI) allows th
 e production of desolvated ions from proteins and protein complexes in sol
 ution. Analysis of these gaseous biomolecules by mass spectrometry  revea
 ls the nature of protein interaction partners. Ion mobility spectrometry a
 nd various dissociation experiments provide additional insights. This pres
 entation will focus on two research directions that are being pursued in t
 he Konermann laboratory. At the very “fundamental” end of the spectrum
 \, we conduct experiments and molecular dynamics simulations to uncover th
 e ESI mechanism\, and for understanding how proteins respond to the transi
 tion from solution into the gas phase. Our work also has a more “applied
 ” side\, where hydrogen/deuterium exchange techniques are being used to 
 decipher the inner workings of proteins in solution. As an example\, we wi
 ll highlight recent experiments on ATP synthase\, the world’s smallest r
 otational motor.\nSelected Publications:\n"Load-dependent destabilization 
 of the γ-rotor shaft in FOF1 ATP synthase revealed by H/D-exchange mass s
 pectrometry" S. Vahidi\, Y. Bi\, S. D. Dunn\, and L. Konermann PNAS in pre
 ss (2016).\n"Release of Native-Like Gaseous Proteins from Electrospray Dro
 plets via The Charged Residue Mechanism: Insights from Molecular Dynamics 
 Simulations" R. G. McAllister\, H. Metwally\, Y. Sun\, and L. Konermann JA
 CS 137\, 12667 (2015).\n"Molecular Dynamics Simulations of the Electrospra
 y Process: Formation of NaCl Clusters via the Charged Residue Mechanism" L
 . Konermann\, R. G. McAllister\, H. Metwally J. Phys. Chem. B 118\, 12025 
 (2014).\n"Effects of Protein-Ligand Interactions on Hydrogen/Deuterium Exc
 hange Kinetics: Canonical and Non-Canonical Scenarios" M. A. Sowole and L.
  Konermann Anal. Chem. 86\, 6715 (2014).\n"Hydrogen Exchange Mass Spectrom
 etry of Bacteriorhodopsin Reveals Light-Induced Changes in the Structural 
 Dynamics of a Biomolecular Machine" Y. Pan\, L. Brown\, and L. Konermann J
 ACS 133\, 20237 (2011).\n"H/D Exchange Mass Spectrometry with Top-Down Ele
 ctron Capture Dissociation for Characterizing Structural Transitions of a 
 17 kDa Protein" J. Pan\, J. Han\, C. H. Borchers\, and L. Konermann JACS 1
 31\, 12801 (2009).
LOCATION:CH G1 495 https://plan.epfl.ch/?room==CH%20G1%20495
STATUS:CONFIRMED
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