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SUMMARY:Super-Resolution Microscopy with DNA Molecules
DTSTART:20160308T141500
DTSTAMP:20260407T162035Z
UID:86d4ad2ec1b3e7e5c6966d2c89b301f4143d8d3d8397bb9b01bfedf2
CATEGORIES:Conferences - Seminars
DESCRIPTION:Ralf Jungmann\, Ph.D.\, Max Planck Institute of Biochemistry\,
  Munich (D)\nBIOENGINEERING SEMINARAbstract:\nSuper-resolution fluorescenc
 e microscopy is a powerful tool for biological research\, but obtaining mu
 ltiplexed images for a large number of distinct target species in whole ce
 lls and beyond remains challenging. Here we use the transient binding of s
 hort fluorescently labeled oligonucleotides (DNA-PAINT\, a variation of po
 int accumulation for imaging in nanoscale topography) for simple and easy-
 to-implement multiplexed super-resolution imaging that achieves sub-10-nm 
 spatial resolution in vitro on synthetic DNA structures.\nWe report a mult
 iplexing approach (Exchange-PAINT) that allows sequential imaging of multi
 ple targets using only a single dye and a single laser source. We experime
 ntally demonstrate ten-color super-resolution imaging in vitro on syntheti
 c DNA structures as well as four-color two-dimensional imaging and three-c
 olor 3D imaging of proteins in fixed cells.\nFinally\, we demonstrate whol
 e cell imaging using DNA- and Exchange-PAINT and optical sectioning\, now 
 allowing DNA-based super-resolution imaging deep inside cells\, away from 
 the glass coverslip.Bio:\nSince 2014  Independent group leader at the Max
  Planck Institute of Biochemistry and Ludwig-Maximilians-Universität Mün
 chen\n2011 - 2014  Postdoctoral researcher at the Wyss Institute for Biol
 ogically Inspired Engineering at Harvard University in the groups of Prof.
  Dr. Peng Yin and Prof. Dr. William M Shih\n2010 - 2011  Postdoctoral res
 earcher at Technische Universität München in the group of Prof. Dr. Frie
 drich C. Simmel\n2007 - 2010  Ph.D. with Prof. Dr. Friedrich C. Simmel at
  Technische Universität München\n2005 - 2006  Diploma Thesis work with 
 Prof. Dr. Paul K Hansma at University of California Santa Barbara\n2001 - 
 2006  Studies in physics at Saarland University
LOCATION:SV1717.1 http://map.epfl.ch/?room=sv1717.1
STATUS:CONFIRMED
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