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SUMMARY:"Differentiation of ES cells into pancreatic and hepatic cells"
DTSTART:20090713T121500
DTSTAMP:20260924T195658Z
UID:0ed929f7f9566ce53d166b1be5b8bc883d65d18cf6a41969bcea26eb
CATEGORIES:Conferences - Seminars
DESCRIPTION:Prof. Shoen Kume\,  Kumamoto University\, Department of Stem C
 ell Biology\nThe endoderm gives rise to the respiratory and digestive orga
 ns such as lung\, liver\, pancreas and intestine\, which are therapeutical
 ly useful.  Here I show that ES cells cultured on M15 cells\, a supportive
  cell line of mesonephros origin\, are efficiently differentiated into an 
 endodermal fate\, and then adopted fates of various digestive organs such 
 as pancreatic and hepatic lineages. Under culture condition for pancreatic
  differentiation\, approximately 31% of the ES cells were induced to diffe
 rentiate to Pdx1-expressing cells. Transplantation studies demonstrate the
  potential of these all pancreatic lineages\, such as endocrine\, exocrine
 Pdx1+ cells to turn into  and duct cells.  Under condition for hepatic 
 differentiation\, the expression of AFP-\, Albumin-\, DBA- positive cells 
 appeared in a sequential manner\, and other markers associated with mature
  hepatic cells appeared after a prolonged culture.\n  Using a synthesized 
 basement membrane (sBM) substratum and the modification of culture media\,
  ES cells could be selectively differentiated into a pancreatic fate. IPS 
 cells could also be induced to pancreatic cells using the same protocol as
  ES cells upon culture on sBM substrates. These results suggest that basem
 ent membrane play a role to promote differentiation.\n  In conclusion\, we
  have established an in vitro differentiation procedure to direct ES cell 
 differentiation into pancreatic and hepatic lineages. This novel procedure
  for endoderm differentiation will be useful for elucidation of molecular 
 mechanisms of gut regionalization and could represent an attractive approa
 ch for a surrogate cell source for not only regenerative medicine\, but al
 so pharmaceutical studies such as toxicology.
LOCATION:SV 1717A
STATUS:CONFIRMED
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