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SUMMARY:SPR biosensing using graphene-based chips
DTSTART:20160531T100000
DTSTAMP:20260407T021915Z
UID:98b63dd07f01be39e2d74a211d87f07ac1ed2d04fbb802b8222eb1a9
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr. Yury Stebunov\nMoscow Institute of Physics and Technology\
 , Russia\nGraphene and graphene oxide are opening up many new opportunitie
 s for biosensing\napplications. The hexagonal lattice structure of graphen
 e and its derivatives allows its interaction\nwith a wide range of biologi
 cal substances via pi-stacking. In addition\, graphene oxide possesses\ndi
 fferent oxygen-containing functional groups for the covalent immobilizatio
 n of biomolecules.\nMoreover\, reduction of graphene oxide can finely tune
  chemical\, electrical and optical properties of\ncarbon material for spec
 ific biosensing applications. The major advantage of graphene and its\nder
 ivatives for biosensing applications is the extremely high surface area of
  different structures\nmade from these materials\, which provides high imm
 obilization efficiency for a wide range of\nbiologically significant subst
 ances such as DNAs\, RNAs\, proteins\, including antibodies and\nmembrane 
 proteins\, viruses\, and bacteria. Here\, we describe a novel type of grap
 hene oxide\nlinking layer for highly sensitive biosensing based on surface
  plasmon resonance (SPR)\, which\nhas become an indispensable tool for sci
 entific research and drug development [1]. During the last\nthree decades\
 , researchers have used only two technologies of linking layers for SPR bi
 osensors\,\nwhich are based on self-assembled monolayers of thiol molecule
 s and on hydrogel layers. Using\ngraphene and its derivatives\, we develop
 ed biosensor chips for existing commercial biosensors\,\nwhose sensitivity
  is higher than for commercial sensor chips available on the market [2] (F
 ig. 1-2).\nModification of carboxyl groups to N-hydroxysuccinimide esters 
 in the flow cell of SPR biosensor\ndemonstrated that the number of carboxy
 l groups\, which can be used for molecule immobilization\,\nis more than 2
 0 times higher in the graphene oxide linking layer than in the linking lay
 er of\ncommercial hydrogel-based sensor chip. In addition\, the graphene o
 xide sensor chip was\ndemonstrated to be 3 times more sensitive comparing 
 to the commercial hydrogel chips when\nusing in the standard biosensing pr
 otocol based on streptavidin-biotin interaction with streptavidin\nimmobil
 ized on the GO surface via pi-stacking. In conclusion\, SPR biosensor chip
 s based on\ngraphene and its derivatives have higher sensitivity comparing
  to commercially available chips\nbased on hydrogels. This will enable us 
 to investigate interactions of protein targets with small\nligands and wil
 l broad-en and accelerate academic and pharmaceutical research.\n[1] Y.V. 
 Stebunov\, O.A. Aftenieva\, A.V. Arsenin\, V.S. Volkov\, ACS Appl. Mat. In
 terfaces 7\, 21727-\n21734 (2015).\n[2] A.V. Arsenin\, Yu.V. Stebunov. RU 
 Patent Application No. 2527699 (Feb 2013)\; US Patent\nApplication No. 201
 50301039 (Oct 2015).
LOCATION:BSP 318
STATUS:CONFIRMED
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