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SUMMARY:Protein complex formation during starch digestion in the human mic
 robiome
DTSTART:20160726T121500
DTSTAMP:20260412T110514Z
UID:8aeafd2ca75366c2b630004b4586e6b361cf8912d037a650bad92482
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr. Hannah Tuson\, Department of Chemistry\, University of Mic
 higan\nBio: I am using single-molecule fluorescence microscopy to examine 
 the localization and dynamics of proteins in live bacteria. I work primari
 ly in three biological systems:\n1. the starch utilization system (Sus) in
  the human gut bacterium Bacteroides thetaiotaomicron\, which binds to and
  degrades starch\;\n2. the proteins involved in replication restart after 
 fork collapse in Bacillus subtilis\; and\n3. the TonB-dependent iron impor
 ter FhuA in Escherichia coli.\nBacteroides thetaiotaomicron is one of the 
 most abundant inhabitants of the mammalian gut. This obligate anaerobe unl
 ocks nutrients from our diet via the catabolism of host indigestible carbo
 hydrates. This process occurs via substrate-specific polysaccharide utiliz
 ation systems\, of which the best studied is the Starch Utilization System
  (Sus). Sus includes five outer membrane proteins: SusDEF cooperate to bin
 d large starch molecules\, which SusG degrades into smaller oligosaccharid
 es for transport to the periplasm through the SusC porin. We have examined
  the dynamics of SusG in live B. thetaiotaomicron cells with single-molecu
 le fluorescence microscopy and tracking\, and found that the dynamics of S
 usG are altered in the presence of starch. We are now extending our studie
 s to fluorescently labeled SusE and SusF in order to directly probe the as
 sembly dynamics of the full Sus complex. We show that both SusE-PAmCherry 
 and SusF-PAmCherry are essentially stationary in the membrane\, even when 
 other members of the Sus complex are absent. Additionally\, we show that m
 obile SusG often co-localizes with stationary SusE and SusF. This research
  will allow us to better understand the role of the Sus proteins in starch
  breakdown by symbiotic bacteria during normal human digestion\, as well a
 s to discern the process of carbohydrate metabolism by gut bacteria in gen
 eral.
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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