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SUMMARY:Fluidic Force Microscopy: a Versatile Technology for Single-Cell B
 iology
DTSTART:20160908T110000
DTSTAMP:20260407T105352Z
UID:cd2421728318dad333338236b25c75da4019fff3eb5e244ea3c99577
CATEGORIES:Conferences - Seminars
DESCRIPTION:Orane Guillaume-Gentil\, Ph.D.\, ETHZ\, Zürich (CH)\nBIOENGIN
 EERING SEMINAR\n\nAbstract:\nWhile single-cell studies can provide unique 
 insights into cellular function\, cell-cell communication\, and cell respo
 nse to external stimuli\, innovative approaches are required to achieve ta
 ilored perturbations and analyses with single-cell resolution. In this res
 pect\, we are developing novel strategies to selectively perturb\, isolate
 \, or analyse single cells within physiological environments\, using fluid
 ic force microscopy (FluidFM). FluidFM combines atomic force microscopy wi
 th microfluidics via microchanneled cantilevers with nano-sized apertures.
  The cantilever probes can be positioned with nanometre precision and inte
 ract with targeted cells with piconewton sensitivity\, whereas pressure co
 ntrol through the microchannel enables liquid handling with femtolitre res
 olution. We demonstrated a broad spectrum of single-cell applications\, in
 cluding the delivery of bioactive compounds (enzymes\, DNA\, drugs) or eve
 n microorganisms such as single viruses onto or into selected cells within
  confluent monolayers. Recently\, we achieved non-destructive sampling of 
 tunable fractions of cytoplasm or nucleoplasm from single live cells for s
 ubsequent molecular imaging\, enzyme activity assay\, and transcriptional 
 analyses. The developed FluidFM-based approaches open unprecedented opport
 unities to study cellular dynamics and cell-cell communication with single
 -cell resolution under physiological conditions.\n\nBio:\n2006 – 2010\nP
 hD Student\, ETH Zürich\, Laboratory of Biosensors & Bioelectronics\nFebr
 uary 2011 – present\nPost Doc\, ETH Zurich\, Institute of Microbiology 
  
LOCATION:AI 1 153 http://map.epfl.ch/?room=ai1153
STATUS:CONFIRMED
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