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SUMMARY:Molecular Basis of Limb Loss in Snakes
DTSTART:20160916T150000
DTSTAMP:20260502T063100Z
UID:c7406289a1a325e4ae83f1b2eb907a91c96bad8fd5063adf56128d34
CATEGORIES:Conferences - Seminars
DESCRIPTION:Evgeny Z. Kvon\, Ph.D.\, Lawrence Berkeley National Laborator
 y\, Berkeley\, CA (USA)\nBIOENGINEERING SEMINAR\n\nAbstract:\nThe evolutio
 n of body shape in vertebrates is tightly coupled to changes within cis-
 regulatory sequences\, yet most examples are associated with relatively s
 ubtle morphological alterations. We identified snake-specific sequence c
 hanges in an otherwise highly conserved enhancer that are critical for li
 mb development. The in vivo activity pattern of the enhancer is conserv
 ed across a wide range of vertebrates from fish to human\, but not in sna
 kes. CRISPR/Cas9-mediated replacement of the mouse enhancer with the ort
 holog from human or fish results in normal limb development. In contrast\
 , replacement with a snake ortholog causes severe limb truncation. Synthe
 tic resurrection of a transcription factor binding site that was specifi
 cally lost in the snake lineage restored full enhancer function and rescu
 ed limb formation. Our results provide an example of regulatory sequence 
 changes associated with a major body plan transition and demonstrate the
  conservation of enhancer function over vast evolutionary distances.\n\nB
 io:\n2008 – 2014\nPhD Student\, Research Institute of Molecular Patholog
 y\, Vienna (AT)\n2014 – present\nPostdoctoral Fellow\, Lawrence Berkeley
  National Laboratory    
LOCATION:AI 1 153 http://map.epfl.ch/?room=ai1153
STATUS:CONFIRMED
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