BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Memento EPFL//
BEGIN:VEVENT
SUMMARY:DNA mapping using super resolution fluorescence microscopy
DTSTART:20161102T180000
DTSTAMP:20260407T105854Z
UID:caa20fffa541f601083fcaeb6eb14a418ef4ed252963d86857244f43
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr Jochem Deen (LOB/LBEN)\nYou are cordially invited to the mo
 nthly Pizza-Optics-Beer (POB) seminar\, organized by the EPFL Photonics Ch
 apter (EPC). Please confirm your participation in the doodle. Abstract:  D
 NA mapping is a method to locate the physical location of a small sequence
  on a DNA molecule. In the old days this DNA map was used as a framework f
 or DNA sequencing. Back then\, the method consisted of treating the DNA wi
 th a restriction enzyme that cuts the DNA at specific small sequences and 
 reading out the pattern with a gel or a fluorescence microscope. However\,
  this approach was fundamentally limited in resolution and has been used l
 ess and less over the years. In my research I focus on a the attachment of
  fluorescent molecules to the DNA rather than cutting it. The position of 
 the label can then be determined using super-resolution fluorescence micro
 scopy techniques. This could allow for a far higher density of labels and 
 resolution of localisation. The end result of this approach will be a sequ
 ence-specific pattern visible on the microscope. The ultimate goal is to u
 se this pattern and match it to known sequences in order to determine the 
 species from which the DNA molecule originates. This would be especially u
 seful for the recognition of bacterial species\, which can now only be app
 roximately categorized with current techniques.
LOCATION:cm 1105
STATUS:CONFIRMED
END:VEVENT
END:VCALENDAR