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SUMMARY:<b>“The road to animal-wide relative quantification of proteins 
 by SILAC and LC-MS”</b>
DTSTART:20090115T121500
DTSTAMP:20260916T043418Z
UID:bbb6b59c423c64532a2efd091e38527afddde916796a66760b88c745
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr.Marcus Krüger\, Max Planck Institute for Heart and Lung Re
 search (Bad Nauheim\, Germany)\nThis seminar is part of the regular series
  of IBI cross-disciplinary seminars and is co-organized by Prof. Auwerx gr
 oup and the EPFL-Proteomics Core Facility.\n \nShort Abstract: SILAC (stab
 le isotope labeling of amino acids in cell culture) has unique advantages 
 for quantitative and functional proteomics because of its inherent accurac
 y of quantitation and the ease of interpretation of mass-spectrometry (MS)
  results. In a SILAC experiment two cell populations are generated\, one i
 n a medium that contains the natural amino acid (i.e. 12C6-lysine) and the
  other in a medium that contains the heavy isotope-substituted version (i.
 e. 13C6-lysine). This allows direct comparison of protein expression level
 s by mixing the non-labeled ‘light’ and labeled ‘heavy’ cell popul
 ations. To date SILAC-labeling has been focused mainly to cell culture or 
 microorganisms. I will talk about whether and to what extent whole mice ca
 n be labeled by SILAC. Furthermore\, I will talk about the estimation of p
 rotein turnover rates and the analysis of knock-out mouse proteomes. The f
 ocus will be on the analysis of blood cells lacking expression of b1 integ
 rin or integrin tail binding protein Kindlin-3. I will show that the absen
 ce of Kindlin-3 results in structural defects of erythrocytes.\n\nThe semi
 nar is a unique opportunity to learn about what modern quantitative proteo
 mics techniques like SILAC can bring to the study of knock-out mouse pheno
 types at the protein expression level.\n\nPeople interested in meeting Dr.
  Krüger in the afternoon of the 15th or on the 16th are welcome to contac
 t Marc Moniatte or Prof. Auwerx.
LOCATION:SG 0211 https://plan.epfl.ch/?room==SG%0200211
STATUS:CONFIRMED
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