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SUMMARY:Optical methods for rapid diagnosis in microbiology
DTSTART:20170110T111500
DTSTAMP:20260413T235551Z
UID:0356dc6e2f63ac0553eefb98d1c639f2a97b3dd322b650c1f25c634c
CATEGORIES:Conferences - Seminars
DESCRIPTION:Pierre R. Marcoux CEA\, LETI\, Division Microtechnologies for 
 Biology and Healthcare\, France\nToday\, rapid detection\, identification
  and antibiotic susceptibility testing of bacteria in microbiological diag
 nosis is a major issue. Methods relying on the observation of few or singl
 e bacteria will allow much faster delivery of clinical information. I will
  report three methods studied at CEA Grenoble regarding the characterisati
 on of bacterial pathogens: \n- Rapid enumeration of bacteria: water-in-oi
 l emulsions were used to encapsulate single E. coli cells into picolitre-s
 ized microreactor droplets. Then their enzymatic activity was monitored in
 dividually through time-lapse fluorescence imaging.\n- Rapid and non-invas
 ive detection of pathogens: Nanoporous glasses are used to trap volatile b
 yproducts of bacterial metabolism. The trapping of these volatile compound
 s induces changes of the optical properties of glasses\, either absorbance
  or fluorescence\, so that pathogens can be detected in a non-invasive way
 .\n- Rapid and label-free identification of pathogens: Incident laser ligh
 t on bacterial colonies yields a complex pattern due to optical scattering
 \, either forward or backward. The analysis of these scattergrams through 
 pattern recognition algorithms brings an innovative way of identifying unk
 nown bacteria\, in a non-destructive and non-invasive method
LOCATION:PH L1 503 (Aquarium )
STATUS:CONFIRMED
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