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SUMMARY:iSCAT – a new approach for visualizing biological structures and
  dynamics
DTSTART:20170201T163000
DTEND:20170201T173000
DTSTAMP:20260407T194849Z
UID:bd79e564929428a59f781f8193756498e59aa2ec155d51ad6e81943f
CATEGORIES:Conferences - Seminars
DESCRIPTION:Dr. Joanna Andrecka\, University of Oxford\nAbstract:\nDramati
 c developments in fluorescence microscopy over the past decades have enabl
 ed routine studies down to the single molecule level and structural observ
 ations far beyond the limits defined by diffraction. Despite its many adva
 ntages\, the fundamental limitation of fluorescence detection is the frequ
 ency with which photons can be emitted and thus detected. The result is a 
 considerable gap between the rate at which dynamics can be recorded and th
 e underlying speed of motion on the nanoscale. iSCAT (interferometric scat
 tering microscopy) is an alternative approach that relies on efficient det
 ection of light scattering. iSCAT is capable of following the motion of na
 noscopic labels (e.g. gold nanoparticles) with true nanometer precision do
 wn to the microsecond regime. Using this technique has enabled us to addre
 ss a surprising variety of fundamental questions in biophysics.\nFirst\, I
  will present an example of using iSCAT for single particle tracking\; the
  technique was used in order to follow myosin-5 motion. By precise trackin
 g of the head of myosin-5\, a rotation of its N-terminal domain during the
  power stroke was revealed. Moreover\, it was shown that\, in contradictio
 n to the three-dimensional Brownian search hypothesis\, the detached head 
 occupies a single off-actin transient state and reaches the desired bindin
 g site in a highly controlled manner. Second\, I will show that iSCAT can 
 be used for ultra-sensitive imaging of biological structures such as lipid
  vesicles\, lipid membranes or filaments without any need for labelling. T
 he label free approach can be extended all the way down to the single prot
 ein level and I will present a movie of unlabeled myosin 5 moving along an
  actin filament. Finally\, I will introduce a new concept of using iSCAT\,
  in combination with fluorescence detection and “classic” structural b
 iology methods\, to understand how structural changes in macromolecular ma
 chines enable and regulate their cellular functions.\n 
LOCATION:PH H3 31 https://plan.epfl.ch/?room==PH%20H3%2031
STATUS:CONFIRMED
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