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SUMMARY:BMI Seminar // Harnessing microfluidics in few-cell and single-cel
 l manipulations for biological discovery
DTSTART:20170628T121500
DTEND:20170628T131500
DTSTAMP:20260510T235213Z
UID:9f056089de4e4e4a4e0dc1ff57693710ddd0c239fa13b88e287a58f5
CATEGORIES:Conferences - Seminars
DESCRIPTION:Angela Wu\,  Division of Life Science\, HKUST - The Hong Kong
  University of Science and Technology\, China\nMicrofluidic manipulation i
 n small fluid volumes can both increase assay sensitivities and improve re
 producibility through automation. In my work\, I apply microfluidics to si
 ngle cell analysis\, I systematically evaluate the sensitivity\, precision
 \, and accuracy of various approaches to single-cell RNA-seq.  Our result
 s show that it is possible to use single-cell RNA-seq to perform quantitat
 ive transcriptome measurements of single cells\, and that microfluidic vol
 umes help to reduce result bias. We subsequently used microfluidic-based R
 NA-seq to profile single cells of the developing lung in embryonic mice\, 
 as well as cells of primary human colon and cervix samples\, and identifie
 d novel transcriptional markers for various subpopulations.
LOCATION:SV 1717 https://plan.epfl.ch/?room==SV%201717
STATUS:CONFIRMED
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